Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes

Enrico Iaccino, Selena Mimmi, Vincenzo Dattilo, Fabiola Marino, Patrizio Candeloro, Antonio Di Loria, Danilo Marimpietri, Antonio Pisano, Francesco Albano, Eleonora Vecchio, Simona Ceglia, Gaetanina Golino, Antonio Lupia, Giuseppe Fiume, Ileana Quinto, Giuseppe Scala

Research output: Contribution to journalArticle

Abstract

Tumor-derived exosomes (TDEs) play a pivotal role in tumor establishment and progression, and are emerging biomarkers for tumor diagnosis in personalized medicine. To date, there is a lack of efficient technology platforms for exosome isolation and characterization. Multiple myeloma (MM) is an incurable B-cell malignancy due to the rapid development of drug-resistance. MM-released exosomes express the immunoglobulin B-cell receptor (Ig-BCR) of the tumor B-cells, which can be targeted by Idiotype-binding peptides (Id-peptides). In this study, we analyzed the production of MM-released exosomes in the murine 5T33MM multiple myeloma model as biomarkers of tumor growth. To this end, we selected Id-peptides by screening a phage display library using as bait the Ig-BCR expressed by 5T33MM cells. By FACS, the FITC-conjugated Id-peptides detected the MM-released exosomes in the serum of 5T33MM-engrafted mice, levels of which are correlated with tumor progression at an earlier time point compared to serum paraprotein. These results indicate that Id-peptide-based recognition of MM-released exosomes may represent a very sensitive diagnostic approach for clinical evaluation of disease progression.

Original languageEnglish
Pages (from-to)159
JournalMolecular Cancer
Volume16
Issue number1
DOIs
Publication statusPublished - Oct 13 2017

Keywords

  • Journal Article

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    Iaccino, E., Mimmi, S., Dattilo, V., Marino, F., Candeloro, P., Di Loria, A., Marimpietri, D., Pisano, A., Albano, F., Vecchio, E., Ceglia, S., Golino, G., Lupia, A., Fiume, G., Quinto, I., & Scala, G. (2017). Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes. Molecular Cancer, 16(1), 159. https://doi.org/10.1186/s12943-017-0730-8