Abstract
Objective: Absolute CD4+ T cell count and human cytomegalovirus (HCMV)-specific CD4+ T cell frequency (as determined by cytokine flow cytometry, CFC) were compared for their ability to predict HCMV disease and safe discontinuation of HCMV secondary prophylaxis. Study design: Three groups of AIDS patients with previous nadir CD4+ T cell count + T cell count (group C). Results: During follow-up, while some patients showed a stable HCMV-specific CD4+ T cell response, others had a fluctuating response (unstable responders) or showed no response at all. In detail, 13/48 group A patients were either HCMV non-responders or unstable responders and 2 of them developed HCMV viremia; 3/11 group B patients were unstable responders, none developing either HCMV viremia or disease; finally, 9 group C patients discontinued HCMV prophylaxis based on absolute CD4+ T cell count >150cells/μl, but in 2 of them lacking HCMV-specific response HCMV retinitis relapsed. None of the seven group C patients discontinuing HCMV prophylaxis on the basis of CFC showed HCMV disease relapse. Conclusions: CFC may support absolute CD4+ T cell count for both guiding HCMV prophylaxis discontinuation and better monitoring HCMV infection in AIDS patients with no previous HCMV disease or having discontinued HCMV prophylaxis.
| Original language | English |
|---|---|
| Pages (from-to) | 297-307 |
| Number of pages | 11 |
| Journal | Journal of Clinical Virology |
| Volume | 29 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2004 |
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Keywords
- AIDS
- CD4 T cells
- Cytokine flow cytometry
- HAART
- HCMV disease
- HCMV prophylaxis
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Monitoring of human cytomegalovirus (HCMV)-specific CD4+ T cell frequency by cytokine flow cytometry as a possible indicator for discontinuation of HCMV secondary prophylaxis in HAART-treated AIDS patients. / Lilleri, Daniele; Piccinini, Giampiero; Genini, Emilia; Comolli, Giuditta; Chiesa, Antonella; Tordato, Federica; Sotgiu, Giovanni; Parisi, Aldo; Baldanti, Fausto; Revello, Maria Grazia; Gerna, Giuseppe.
In: Journal of Clinical Virology, Vol. 29, No. 4, 04.2004, p. 297-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monitoring of human cytomegalovirus (HCMV)-specific CD4+ T cell frequency by cytokine flow cytometry as a possible indicator for discontinuation of HCMV secondary prophylaxis in HAART-treated AIDS patients
AU - Lilleri, Daniele
AU - Piccinini, Giampiero
AU - Genini, Emilia
AU - Comolli, Giuditta
AU - Chiesa, Antonella
AU - Tordato, Federica
AU - Sotgiu, Giovanni
AU - Parisi, Aldo
AU - Baldanti, Fausto
AU - Revello, Maria Grazia
AU - Gerna, Giuseppe
PY - 2004/4
Y1 - 2004/4
N2 - Objective: Absolute CD4+ T cell count and human cytomegalovirus (HCMV)-specific CD4+ T cell frequency (as determined by cytokine flow cytometry, CFC) were compared for their ability to predict HCMV disease and safe discontinuation of HCMV secondary prophylaxis. Study design: Three groups of AIDS patients with previous nadir CD4+ T cell count + T cell count (group C). Results: During follow-up, while some patients showed a stable HCMV-specific CD4+ T cell response, others had a fluctuating response (unstable responders) or showed no response at all. In detail, 13/48 group A patients were either HCMV non-responders or unstable responders and 2 of them developed HCMV viremia; 3/11 group B patients were unstable responders, none developing either HCMV viremia or disease; finally, 9 group C patients discontinued HCMV prophylaxis based on absolute CD4+ T cell count >150cells/μl, but in 2 of them lacking HCMV-specific response HCMV retinitis relapsed. None of the seven group C patients discontinuing HCMV prophylaxis on the basis of CFC showed HCMV disease relapse. Conclusions: CFC may support absolute CD4+ T cell count for both guiding HCMV prophylaxis discontinuation and better monitoring HCMV infection in AIDS patients with no previous HCMV disease or having discontinued HCMV prophylaxis.
AB - Objective: Absolute CD4+ T cell count and human cytomegalovirus (HCMV)-specific CD4+ T cell frequency (as determined by cytokine flow cytometry, CFC) were compared for their ability to predict HCMV disease and safe discontinuation of HCMV secondary prophylaxis. Study design: Three groups of AIDS patients with previous nadir CD4+ T cell count + T cell count (group C). Results: During follow-up, while some patients showed a stable HCMV-specific CD4+ T cell response, others had a fluctuating response (unstable responders) or showed no response at all. In detail, 13/48 group A patients were either HCMV non-responders or unstable responders and 2 of them developed HCMV viremia; 3/11 group B patients were unstable responders, none developing either HCMV viremia or disease; finally, 9 group C patients discontinued HCMV prophylaxis based on absolute CD4+ T cell count >150cells/μl, but in 2 of them lacking HCMV-specific response HCMV retinitis relapsed. None of the seven group C patients discontinuing HCMV prophylaxis on the basis of CFC showed HCMV disease relapse. Conclusions: CFC may support absolute CD4+ T cell count for both guiding HCMV prophylaxis discontinuation and better monitoring HCMV infection in AIDS patients with no previous HCMV disease or having discontinued HCMV prophylaxis.
KW - AIDS
KW - CD4 T cells
KW - Cytokine flow cytometry
KW - HAART
KW - HCMV disease
KW - HCMV prophylaxis
UR - http://www.scopus.com/inward/record.url?scp=10744223665&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10744223665&partnerID=8YFLogxK
U2 - 10.1016/S1386-6532(03)00214-2
DO - 10.1016/S1386-6532(03)00214-2
M3 - Article
C2 - 15018859
AN - SCOPUS:10744223665
VL - 29
SP - 297
EP - 307
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 4
ER -