TY - JOUR
T1 - Monitoring of human cytomegalovirus-specific CD4+ and CD8 + T-cell immunity in patients receiving solid organ transplantation
AU - Gerna, G.
AU - Lilleri, D.
AU - Fornara, C.
AU - Comolli, G.
AU - Lozza, L.
AU - Campana, C.
AU - Pellegrini, C.
AU - Meloni, F.
AU - Rampino, T.
PY - 2006/10
Y1 - 2006/10
N2 - Absolute and human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T-cell counts were monitored in 38 solid organ (20 heart, 9 lung and 9 kidney) transplant recipients during the first year after transplantation by a novel assay based on T-cell stimulation with HCMV-infected autologous dendritic cells. According to the pattern of T-cell restoration occurring either within the first month after transplantation or later, patients were classified as either early (n = 21) or late responders (n = 17). HCMV-specific CD4 + and CD8+ T-cell counts were consistently lower in late compared to early responders from baseline through 6 months after transplantation. In addition, in late responders, while HCMV infection preceded immune restoration, HCMV-specific CD4+ restoration was significantly delayed with respect to CD8+ T-cell restoration. The number of HCMV-specific CD4+ and CD8+ T-cells detected prior to transplantation significantly correlated with time to T-cell immunity restoration, in that higher HCMV-specific T-cell counts predicted earlier immune restoration. Clinically, the great majority of early responders (18/21, 85.7%) underwent self-resolving HCMV infections (p = 0.004), whereas the great majority of late responders (13/17, 76.5%) were affected by HCMV infections requiring antiviral treatment (p =
AB - Absolute and human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T-cell counts were monitored in 38 solid organ (20 heart, 9 lung and 9 kidney) transplant recipients during the first year after transplantation by a novel assay based on T-cell stimulation with HCMV-infected autologous dendritic cells. According to the pattern of T-cell restoration occurring either within the first month after transplantation or later, patients were classified as either early (n = 21) or late responders (n = 17). HCMV-specific CD4 + and CD8+ T-cell counts were consistently lower in late compared to early responders from baseline through 6 months after transplantation. In addition, in late responders, while HCMV infection preceded immune restoration, HCMV-specific CD4+ restoration was significantly delayed with respect to CD8+ T-cell restoration. The number of HCMV-specific CD4+ and CD8+ T-cells detected prior to transplantation significantly correlated with time to T-cell immunity restoration, in that higher HCMV-specific T-cell counts predicted earlier immune restoration. Clinically, the great majority of early responders (18/21, 85.7%) underwent self-resolving HCMV infections (p = 0.004), whereas the great majority of late responders (13/17, 76.5%) were affected by HCMV infections requiring antiviral treatment (p =
KW - HCMV-specific CD4 T cells
KW - HCMV-specific CD8 T cells
KW - Human cytomegalovirus
KW - Monitoring of T-cell immunity
KW - T-cell immunity solid organ transplantation
UR - http://www.scopus.com/inward/record.url?scp=33748494938&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748494938&partnerID=8YFLogxK
U2 - 10.1111/j.1600-6143.2006.01488.x
DO - 10.1111/j.1600-6143.2006.01488.x
M3 - Article
C2 - 16889599
AN - SCOPUS:33748494938
VL - 6
SP - 2356
EP - 2364
JO - American Journal of Transplantation
JF - American Journal of Transplantation
SN - 1600-6135
IS - 10
ER -