Monitoring of low density lipoprotein oxidation by low-level chemiluminescence

Riccardo Albertini, Peter M. Abuja

Research output: Contribution to journalArticle

Abstract

A method for monitoring low-density lipoprotein (LDL) oxidation by low-level chemiluminescence (LL-CL) is described in this study. The kinetic indices obtained with this procedure, in particular lag-time and K value (related to prooxidant activity of Cu2+ bound to LDL) are compared with those of the established UV-absorbing conjugated diene assay. The correlation of lag-time values obtained by LL-CL and conjugated diene assay was very high both in the case of Cu2+- and peroxyl-radical-mediated oxidation (r = 0.99). By using the transient free radical scavenging activity of butylated hydroxytoluene, a calibration of LL-CL for lipid peroxyl radical and termination rate was obtained. The spectral analysis of LL-CL from oxidizing LDL shows a maximum peak between 420 and 500 nm, corresponding to the emission of triplet carbonyl compounds. LL-CL allows continuous and direct monitoring of LDL oxidation as extraction and derivatization of lipid peroxidation products are not required. Moreover, some limitations of UV spectrescopy such as by absorbing compounds need not be considered. Therefore, the present procedure represents a simple and convenient tool for continuous monitoring of LDL oxidation which may be applied to mechanistic and clinical studies.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalFree Radical Research
Volume29
Issue number1
DOIs
Publication statusPublished - 1998

Keywords

  • Lipid peroxidation
  • Low density lipoprotein
  • Low-level chemiluminescence

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Monitoring of low density lipoprotein oxidation by low-level chemiluminescence'. Together they form a unique fingerprint.

  • Cite this