Monitoring the effects of drug treatment in rat models of disease by serum protein analysis

Elisabetta Gianazza, Ivano Eberini, Pia Villa, Maddalena Fratelli, Christian Pinna, Robin Wait, Manfred Gemeiner, Ingrid Miller

Research output: Contribution to journalArticle

Abstract

In this review we list from literature investigations on rat serum proteins using electrophoretic techniques in connection with drug testing. From our own research work, we provide annotated two-dimensional maps of rat serum proteins under control and experimental conditions. Emphasis is on species-specific components and on the effects of acute and chronic inflammation. We discuss our project of structural proteomics on rat serum as a minimally invasive approach to pharmacological investigation, and we outline a typical experimental plan for drug testing according to the above guidelines. We then report in detail on the results of our trials of anti-inflammatory drugs on adjuvant arthritis, an animal model of disease resembling in many aspects human rheumatoid arthritis. We demonstrate a correlation between biochemical parameters and therapeutic findings and outline the advantages of the chosen methodological approach, which proved also sensitive in revealing "side effects" of the test drugs. In an appendix we describe our experimental protocol when performing two-dimensional electrophoresis of rat serum.

Original languageEnglish
Pages (from-to)107-130
Number of pages24
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume771
Issue number1-2
DOIs
Publication statusPublished - May 5 2002

Keywords

  • Drug treatment effects monitoring
  • Proteins
  • Reviews

ASJC Scopus subject areas

  • Biochemistry

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