Monoamine oxidase inhibition prevents mitochondrial dysfunction and apoptosis in myoblasts from patients with collagen VI myopathies

E. Sorato, S. Menazza, A. Zulian, P. Sabatelli, F. Gualandi, L. Merlini, P. Bonaldo, M. Canton, P. Bernardi, F. Di Lisa

Research output: Contribution to journalArticle

Abstract

Although mitochondrial dysfunction and oxidative stress have been proposed to play a crucial role in several types of muscular dystrophy (MD), whether a causal link between these two alterations exists remains an open question. We have documented that mitochondrial dysfunction through opening of the permeability transition pore plays a key role in myoblasts from patients as well as in mouse models of MD, and that oxidative stress caused by monoamine oxidases (MAO) is involved in myofiber damage. In the present study we have tested whether MAO-dependent oxidative stress is a causal determinant of mitochondrial dysfunction and apoptosis in myoblasts from patients affected by collagen VI myopathies. We find that upon incubation with hydrogen peroxide or the MAO substrate tyramine myoblasts from patients upregulate MAO-B expression and display a significant rise in reactive oxygen species (ROS) levels, with concomitant mitochondrial depolarization. MAO inhibition by pargyline significantly reduced both ROS accumulation and mitochondrial dysfunction, and normalized the increased incidence of apoptosis in myoblasts from patients. Thus, MAO-dependent oxidative stress is causally related to mitochondrial dysfunction and cell death in myoblasts from patients affected by collagen VI myopathies, and inhibition of MAO should be explored as a potential treatment for these diseases.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalFree Radical Biology and Medicine
Volume75
DOIs
Publication statusPublished - 2014

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Keywords

  • Cell death
  • Mitochondria
  • Monoamine oxidases
  • Muscular dystrophy
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)
  • Medicine(all)

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