Monoclonal antibodies against Candida rugosa lipase

Hassan Rahimi, Simonetta Soro, Aurelia Rughetti, Cleofe Palocci, Mauro Biffoni, Serena Barachini, Federica Taurino, Enrico Cernia, Luigi Frati, Marianna Nuti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG1 isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalJournal of Molecular Catalysis B: Enzymatic
Volume28
Issue number2-3
DOIs
Publication statusPublished - May 4 2004

Fingerprint

Monoclonal antibodies
Candida
Lipases
Lipase
Enzymes
Monoclonal Antibodies
Antibodies
Immobilization
Fermentation
Purification
Epitopes
Catalyst activity
Protein Isoforms
Immunoglobulin G

Keywords

  • Candida rugosa lipase
  • IgG isotype
  • Microbial lipase

ASJC Scopus subject areas

  • Biochemistry
  • Catalysis
  • Process Chemistry and Technology

Cite this

Rahimi, H., Soro, S., Rughetti, A., Palocci, C., Biffoni, M., Barachini, S., ... Nuti, M. (2004). Monoclonal antibodies against Candida rugosa lipase. Journal of Molecular Catalysis B: Enzymatic, 28(2-3), 71-74. https://doi.org/10.1016/j.molcatb.2004.01.002

Monoclonal antibodies against Candida rugosa lipase. / Rahimi, Hassan; Soro, Simonetta; Rughetti, Aurelia; Palocci, Cleofe; Biffoni, Mauro; Barachini, Serena; Taurino, Federica; Cernia, Enrico; Frati, Luigi; Nuti, Marianna.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 28, No. 2-3, 04.05.2004, p. 71-74.

Research output: Contribution to journalArticle

Rahimi, H, Soro, S, Rughetti, A, Palocci, C, Biffoni, M, Barachini, S, Taurino, F, Cernia, E, Frati, L & Nuti, M 2004, 'Monoclonal antibodies against Candida rugosa lipase', Journal of Molecular Catalysis B: Enzymatic, vol. 28, no. 2-3, pp. 71-74. https://doi.org/10.1016/j.molcatb.2004.01.002
Rahimi H, Soro S, Rughetti A, Palocci C, Biffoni M, Barachini S et al. Monoclonal antibodies against Candida rugosa lipase. Journal of Molecular Catalysis B: Enzymatic. 2004 May 4;28(2-3):71-74. https://doi.org/10.1016/j.molcatb.2004.01.002
Rahimi, Hassan ; Soro, Simonetta ; Rughetti, Aurelia ; Palocci, Cleofe ; Biffoni, Mauro ; Barachini, Serena ; Taurino, Federica ; Cernia, Enrico ; Frati, Luigi ; Nuti, Marianna. / Monoclonal antibodies against Candida rugosa lipase. In: Journal of Molecular Catalysis B: Enzymatic. 2004 ; Vol. 28, No. 2-3. pp. 71-74.
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