Monoclonal antibodies against NIH 3T3 cells transformed by human thyroid carcinoma DNA

R. Alzani, M. A. Pierotti, S. Menard, S. Canevari, M. L. Feronani, D. Ballinari, C. Traversari, G. Della Torre, P. Radice, S. Pilotti, A. Fusco, M. Grieco, G. Della Porta, M. I. Colnaghi

Research output: Contribution to journalArticle

Abstract

First cycle transformants of NIH 3T3 cells transfected with metastatic human thyroid carcinoma DNA were used as immunogen to obtain monoclonal antibodies (MAbs) against normal and transformation-related antigens. The transformed cell line (M33) was shown to contain Alu sequences. Two MAbs were selected on the basis of their differential reactivity toward untreated NIH 3T3 cells or the transformed M33 cell line. By immunofluorescence, immunoelectronmicroscopy and biochemical analysis, the first MAb (MTrl) was demonstrated to recognize an epitope on cytoskeletal filaments of proliferating murine fibroblasts. Similar MTrl-labelled filaments were also found to accumulate into cytoplast-like structures spontaneously produced by M33 cells. The characterization by immunofluorescence of MTr2, the second MAb, indicates that it recognizes a specific human antigen associated with normal thyroid epithelial cells and differentiated thyroid tumors.

Original languageEnglish
Pages (from-to)7-18
Number of pages12
JournalHybridoma
Volume7
Issue number1
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Genetics
  • Immunology

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    Alzani, R., Pierotti, M. A., Menard, S., Canevari, S., Feronani, M. L., Ballinari, D., Traversari, C., Della Torre, G., Radice, P., Pilotti, S., Fusco, A., Grieco, M., Della Porta, G., & Colnaghi, M. I. (1988). Monoclonal antibodies against NIH 3T3 cells transformed by human thyroid carcinoma DNA. Hybridoma, 7(1), 7-18.