Monoclonal antibodies against recombinant human growth hormone as probes to study immune function

D. Lattuada, C. Casnici, S. Gregori, A. Berrini, C. Secchi, P. Franco, O. Marelli

Research output: Contribution to journalArticle

Abstract

Monoclonal antibodies were raised against human recombinant growth hormone (rhGH) and those that did not cross-react with other human recombinant proteins like prolactin (PRL), interleukin 2 (IL-2), insulin, or bovine pituitary growth hormone were selected. The selected hybridoma supernatants were studied for their ability to influence T lymphocyte proliferation when induced either by a mitogen, such as phytohemagglutinin (PHA), or by alloantigen. All supernatants inhibited proliferation. Three MAbs were then purified by several passages on antimouse IgG (or IgM)- agarose columns, and characterized. These MAbs recognized three different epitopes, as revealed by competition study, although their inhibitory effect on PHA-induced T cell proliferation was quite similar. The data demonstrate that the MAbs were not cytolytic, that they did not interfere with the PHA binding to T cell membranes, and, as revealed by FACS analysis, did not bind to the membrane. Finally, these MAbs immunoprecipitated a 44-kDa molecule from PHA-activated T cell-concentrated supernatants. These data indicate that the MAbs recognized a soluble factor that plays a central role in T cell proliferation and that is probably the immune growth hormone.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalHybridoma
Volume15
Issue number3
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Immunology
  • Genetics

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    Lattuada, D., Casnici, C., Gregori, S., Berrini, A., Secchi, C., Franco, P., & Marelli, O. (1996). Monoclonal antibodies against recombinant human growth hormone as probes to study immune function. Hybridoma, 15(3), 211-217.