Monoclonal antibodies against recombinant human growth hormone as probes to study immune function

D. Lattuada, C. Casnici, S. Gregori, A. Berrini, C. Secchi, P. Franco, O. Marelli

Research output: Contribution to journalArticle

Abstract

Monoclonal antibodies were raised against human recombinant growth hormone (rhGH) and those that did not cross-react with other human recombinant proteins like prolactin (PRL), interleukin 2 (IL-2), insulin, or bovine pituitary growth hormone were selected. The selected hybridoma supernatants were studied for their ability to influence T lymphocyte proliferation when induced either by a mitogen, such as phytohemagglutinin (PHA), or by alloantigen. All supernatants inhibited proliferation. Three MAbs were then purified by several passages on antimouse IgG (or IgM)- agarose columns, and characterized. These MAbs recognized three different epitopes, as revealed by competition study, although their inhibitory effect on PHA-induced T cell proliferation was quite similar. The data demonstrate that the MAbs were not cytolytic, that they did not interfere with the PHA binding to T cell membranes, and, as revealed by FACS analysis, did not bind to the membrane. Finally, these MAbs immunoprecipitated a 44-kDa molecule from PHA-activated T cell-concentrated supernatants. These data indicate that the MAbs recognized a soluble factor that plays a central role in T cell proliferation and that is probably the immune growth hormone.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalHybridoma
Volume15
Issue number3
Publication statusPublished - 1996

Fingerprint

Human Growth Hormone
Growth Hormone
Phytohemagglutinins
Monoclonal Antibodies
T-Lymphocytes
Cell Proliferation
Isoantigens
Hybridomas
Mitogens
Recombinant Proteins
Prolactin
Sepharose
Interleukin-2
Immunoglobulin M
Epitopes
Immunoglobulin G
Cell Membrane
Insulin
Membranes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Lattuada, D., Casnici, C., Gregori, S., Berrini, A., Secchi, C., Franco, P., & Marelli, O. (1996). Monoclonal antibodies against recombinant human growth hormone as probes to study immune function. Hybridoma, 15(3), 211-217.

Monoclonal antibodies against recombinant human growth hormone as probes to study immune function. / Lattuada, D.; Casnici, C.; Gregori, S.; Berrini, A.; Secchi, C.; Franco, P.; Marelli, O.

In: Hybridoma, Vol. 15, No. 3, 1996, p. 211-217.

Research output: Contribution to journalArticle

Lattuada, D, Casnici, C, Gregori, S, Berrini, A, Secchi, C, Franco, P & Marelli, O 1996, 'Monoclonal antibodies against recombinant human growth hormone as probes to study immune function', Hybridoma, vol. 15, no. 3, pp. 211-217.
Lattuada D, Casnici C, Gregori S, Berrini A, Secchi C, Franco P et al. Monoclonal antibodies against recombinant human growth hormone as probes to study immune function. Hybridoma. 1996;15(3):211-217.
Lattuada, D. ; Casnici, C. ; Gregori, S. ; Berrini, A. ; Secchi, C. ; Franco, P. ; Marelli, O. / Monoclonal antibodies against recombinant human growth hormone as probes to study immune function. In: Hybridoma. 1996 ; Vol. 15, No. 3. pp. 211-217.
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