TY - JOUR
T1 - Monoclonal antibodies conjugated with superparamagnetic iron oxide particles allow magnetic resonance imaging detection of lymphocytes in the mouse brain
AU - Luchetti, Alessandro
AU - Milani, Davide
AU - Ruffini, Francesca
AU - Galli, Rossella
AU - Falini, Andrea
AU - Quattrini, Angelo
AU - Scotti, Giuseppe
AU - Comi, Giancarlo
AU - Martino, Gianvito
AU - Furlan, Roberto
AU - Politi, Letterio S.
PY - 2012/3
Y1 - 2012/3
N2 - We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T 2* relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220 + cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3 + infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle.
AB - We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T 2* relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220 + cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3 + infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle.
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U2 - 10.2310/7290.2011.00032
DO - 10.2310/7290.2011.00032
M3 - Article
C2 - 22469239
AN - SCOPUS:84860731771
VL - 11
SP - 114
EP - 125
JO - Molecular Imaging
JF - Molecular Imaging
SN - 1535-3508
IS - 2
ER -