Monoclonal antibodies for the different chains of chick type VI collagen.

A. Colombatti, K. Ainger, M. T. Mucignat, P. Bonaldo

Research output: Contribution to journalArticle

Abstract

Avian type VI collagen is composed of three subunits of Mr 140,000, 150,000 and 260,000. Monoclonal antibodies were raised against type VI collagen isolated from chick embryo gizzard, and these antibodies were used to immunoprecipitate type VI collagen from metabolically labeled embryo cells. Several antibodies appeared to react with epitopes independent of glycosylation and hydroxylation processes. The antibody-binding sites were identified on the different chains by immunoblotting of total cell extracts. In addition, antibodies that recognized different epitopes on the Mr 260,000 subunit could be grouped in at least three different clusters by competitive inhibition radioimmunobinding assays.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalCollagen and Related Research
Volume8
Issue number4
Publication statusPublished - Jul 1988

Fingerprint

Collagen Type VI
Monoclonal Antibodies
Antibodies
Epitopes
Antibody Binding Sites
Chick Embryo
Hydroxylation
Cell Extracts
Glycosylation
Immunoblotting
Embryonic Structures

ASJC Scopus subject areas

  • Rheumatology

Cite this

Colombatti, A., Ainger, K., Mucignat, M. T., & Bonaldo, P. (1988). Monoclonal antibodies for the different chains of chick type VI collagen. Collagen and Related Research, 8(4), 331-337.

Monoclonal antibodies for the different chains of chick type VI collagen. / Colombatti, A.; Ainger, K.; Mucignat, M. T.; Bonaldo, P.

In: Collagen and Related Research, Vol. 8, No. 4, 07.1988, p. 331-337.

Research output: Contribution to journalArticle

Colombatti, A, Ainger, K, Mucignat, MT & Bonaldo, P 1988, 'Monoclonal antibodies for the different chains of chick type VI collagen.', Collagen and Related Research, vol. 8, no. 4, pp. 331-337.
Colombatti A, Ainger K, Mucignat MT, Bonaldo P. Monoclonal antibodies for the different chains of chick type VI collagen. Collagen and Related Research. 1988 Jul;8(4):331-337.
Colombatti, A. ; Ainger, K. ; Mucignat, M. T. ; Bonaldo, P. / Monoclonal antibodies for the different chains of chick type VI collagen. In: Collagen and Related Research. 1988 ; Vol. 8, No. 4. pp. 331-337.
@article{c0b834394a54492aa487a5e26568af8d,
title = "Monoclonal antibodies for the different chains of chick type VI collagen.",
abstract = "Avian type VI collagen is composed of three subunits of Mr 140,000, 150,000 and 260,000. Monoclonal antibodies were raised against type VI collagen isolated from chick embryo gizzard, and these antibodies were used to immunoprecipitate type VI collagen from metabolically labeled embryo cells. Several antibodies appeared to react with epitopes independent of glycosylation and hydroxylation processes. The antibody-binding sites were identified on the different chains by immunoblotting of total cell extracts. In addition, antibodies that recognized different epitopes on the Mr 260,000 subunit could be grouped in at least three different clusters by competitive inhibition radioimmunobinding assays.",
author = "A. Colombatti and K. Ainger and Mucignat, {M. T.} and P. Bonaldo",
year = "1988",
month = "7",
language = "English",
volume = "8",
pages = "331--337",
journal = "Collagen and Related Research",
issn = "0174-173X",
publisher = "Gustav Fischer Verlag",
number = "4",

}

TY - JOUR

T1 - Monoclonal antibodies for the different chains of chick type VI collagen.

AU - Colombatti, A.

AU - Ainger, K.

AU - Mucignat, M. T.

AU - Bonaldo, P.

PY - 1988/7

Y1 - 1988/7

N2 - Avian type VI collagen is composed of three subunits of Mr 140,000, 150,000 and 260,000. Monoclonal antibodies were raised against type VI collagen isolated from chick embryo gizzard, and these antibodies were used to immunoprecipitate type VI collagen from metabolically labeled embryo cells. Several antibodies appeared to react with epitopes independent of glycosylation and hydroxylation processes. The antibody-binding sites were identified on the different chains by immunoblotting of total cell extracts. In addition, antibodies that recognized different epitopes on the Mr 260,000 subunit could be grouped in at least three different clusters by competitive inhibition radioimmunobinding assays.

AB - Avian type VI collagen is composed of three subunits of Mr 140,000, 150,000 and 260,000. Monoclonal antibodies were raised against type VI collagen isolated from chick embryo gizzard, and these antibodies were used to immunoprecipitate type VI collagen from metabolically labeled embryo cells. Several antibodies appeared to react with epitopes independent of glycosylation and hydroxylation processes. The antibody-binding sites were identified on the different chains by immunoblotting of total cell extracts. In addition, antibodies that recognized different epitopes on the Mr 260,000 subunit could be grouped in at least three different clusters by competitive inhibition radioimmunobinding assays.

UR - http://www.scopus.com/inward/record.url?scp=0024049884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024049884&partnerID=8YFLogxK

M3 - Article

C2 - 2463889

AN - SCOPUS:0024049884

VL - 8

SP - 331

EP - 337

JO - Collagen and Related Research

JF - Collagen and Related Research

SN - 0174-173X

IS - 4

ER -