Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)

Roberto Burioni, Filippo Canducci, Nicasio Mancini, Nicola Clementi, Monica Sassi, Donata De Marco, Roberta Antonia Diotti, Diego Saita, Michela Sampaolo, Giuseppe Sautto, Matteo Pianezze, Massimo Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

The new H1N1 swine-origin influenza virus (S-OIV) strain is a global health problem. The elucidation of the virus-host relationship is crucial for the control of the new infection. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza strain at very low concentrations were cloned before the emergence of S-OIV from a patient who had a broad-range H1N1 serum neutralizing activity. The two HMabs neutralized all tested H1N1 strains, including S-OIV and a swine strain with IC50 ranging from 2 to 7 μg/ml. Data demonstrate that infection with previously circulating H1N1 strains can elicit antibodies neutralizing S-OIV. Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection.

Original languageEnglish
Pages (from-to)144-152
Number of pages9
JournalVirology
Volume399
Issue number1
DOIs
Publication statusPublished - Mar 30 2010

Keywords

  • Antiviral therapy
  • Immunotherapy
  • Influenza
  • Monoclonal antibodies
  • Oseltamivir
  • Pandemics

ASJC Scopus subject areas

  • Virology

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