Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor

G. Salerno, P. Verde, M. L. Nolli, A. Corti, H. Szöts, T. Meo, J. Johnson, S. Bullock, G. Cassani, F. Blasi

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies have been obtained that recognize either the A or B chain of human urinary urokinase. These antibodies identify human urokinase-producing cells and the product of urokinase mRNA. Anti-urokinase monoclonal antibodies precipitate an ~54,000-dalton protein synthesized in vitro in a rabbit reticulocyte cell-free system. The pro-urokinase appears to be the precursor of both A and B chains of human urinary urokinase. Urokinase mRNA in human kidney constitutes only 0.1% or less of total poly(A)+ RNA.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number1 I
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor'. Together they form a unique fingerprint.

Cite this