Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor

G. Salerno; P. Verde; M. L. Nolli; A. Corti; H. Szöts; T. Meo; J. Johnson; S. Bullock; G. Cassani; F. Blasi

Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor

G. Salerno, P. Verde, M. L. Nolli, A. Corti, H. Szöts, T. Meo, J. Johnson, S. Bullock, G. Cassani, F. Blasi

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Monoclonal antibodies have been obtained that recognize either the A or B chain of human urinary urokinase. These antibodies identify human urokinase-producing cells and the product of urokinase mRNA. Anti-urokinase monoclonal antibodies precipitate an ~54,000-dalton protein synthesized in vitro in a rabbit reticulocyte cell-free system. The pro-urokinase appears to be the precursor of both A and B chains of human urinary urokinase. Urokinase mRNA in human kidney constitutes only 0.1% or less of total poly(A)⁺ RNA.

Original language	English
Pages (from-to)	110-114
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	81
Issue number	1 I
Publication status	Published - 1984

ASJC Scopus subject areas

Genetics
General

Cite this

Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor. / Salerno, G.; Verde, P.; Nolli, M. L.; Corti, A.; Szöts, H.; Meo, T.; Johnson, J.; Bullock, S.; Cassani, G.; Blasi, F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 1 I, 1984, p. 110-114.

Research output: Contribution to journal › Article › peer-review

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AU - Salerno, G.

AU - Verde, P.

AU - Nolli, M. L.

AU - Corti, A.

AU - Szöts, H.

AU - Meo, T.

AU - Johnson, J.

AU - Bullock, S.

AU - Cassani, G.

AU - Blasi, F.

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AB - Monoclonal antibodies have been obtained that recognize either the A or B chain of human urinary urokinase. These antibodies identify human urokinase-producing cells and the product of urokinase mRNA. Anti-urokinase monoclonal antibodies precipitate an ~54,000-dalton protein synthesized in vitro in a rabbit reticulocyte cell-free system. The pro-urokinase appears to be the precursor of both A and B chains of human urinary urokinase. Urokinase mRNA in human kidney constitutes only 0.1% or less of total poly(A)+ RNA.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor

Abstract

ASJC Scopus subject areas

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