Monoclonal antibody 5A binds apolipoprotein B-48 and inhibits the low density lipoprotein-receptor interaction

Alberto Corsini, Simona Fantappiè, Santica Marcovina, Agnese Granata, Remo Fumagalli, Alberico L. Catapano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

in a panel of 10 monoclonal antibodies raised against human LDL we detected three antibodies (named 5A, 6B, and 6E) which recognize both apolipoprotein B-100 and B-48. Antibody 5A inhibited, in a dose dependent manner, the interaction of 125I-LDL with their receptor on human skin fibroblasts. Using thrombolytic fragments, the epitope of antibody 5A was mapped to the carboxy terminal region of apo B-48. MAB 5A was equipotent with MAB Mb 47, an inhibitory antibody whose epitose lies near a putative receptor binding domain of apo B in thrombolytic fragment T2. These findings suggest that areas other than the carboxy terminal portion of apo B-100 may participate in the LDL-receptor interaction, either directly or by determining the exposition of high affinity sites of apo B-100.

Original languageEnglish
Pages (from-to)908-915
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number3
DOIs
Publication statusPublished - Aug 15 1989

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Apolipoprotein B-48
Apolipoprotein B-100
LDL Receptors
Monoclonal Antibodies
Antibodies
Immunoglobulin Fragments
Apolipoproteins B
Epitopes
Fibroblasts
Skin
oxidized low density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Monoclonal antibody 5A binds apolipoprotein B-48 and inhibits the low density lipoprotein-receptor interaction. / Corsini, Alberto; Fantappiè, Simona; Marcovina, Santica; Granata, Agnese; Fumagalli, Remo; Catapano, Alberico L.

In: Biochemical and Biophysical Research Communications, Vol. 162, No. 3, 15.08.1989, p. 908-915.

Research output: Contribution to journalArticle

Corsini, Alberto ; Fantappiè, Simona ; Marcovina, Santica ; Granata, Agnese ; Fumagalli, Remo ; Catapano, Alberico L. / Monoclonal antibody 5A binds apolipoprotein B-48 and inhibits the low density lipoprotein-receptor interaction. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 162, No. 3. pp. 908-915.
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