Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

M. Civilini; C. Pucillo; A. Colombatti; G. Damante; M. De Bertoldi; G. Tell

doi:10.1046/j.1472-765X.2000.00819.x

Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

M. Civilini, C. Pucillo, A. Colombatti, G. Damante, M. De Bertoldi, G. Tell

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article

Abstract

A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 10⁴ colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.

Original language	English
Pages (from-to)	313-318
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	31
Issue number	4
DOIs	https://doi.org/10.1046/j.1472-765X.2000.00819.x
Publication status	Published - 2000

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ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Water Science and Technology
Fluid Flow and Transfer Processes
Microbiology
Ecology

Cite this

Civilini, M., Pucillo, C., Colombatti, A., Damante, G., De Bertoldi, M., & Tell, G. (2000). Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR. Letters in Applied Microbiology, 31(4), 313-318. https://doi.org/10.1046/j.1472-765X.2000.00819.x

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AU - Tell, G.

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10.1046/j.1472-765X.2000.00819.x