Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

M. Civilini, C. Pucillo, A. Colombatti, G. Damante, M. De Bertoldi, G. Tell

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalLetters in Applied Microbiology
Volume31
Issue number4
DOIs
Publication statusPublished - 2000

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Civilini, M., Pucillo, C., Colombatti, A., Damante, G., De Bertoldi, M., & Tell, G. (2000). Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR. Letters in Applied Microbiology, 31(4), 313-318. https://doi.org/10.1046/j.1472-765X.2000.00819.x