Abstract
A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.
| Original language | English |
|---|---|
| Pages (from-to) | 313-318 |
| Number of pages | 6 |
| Journal | Letters in Applied Microbiology |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2000 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Water Science and Technology
- Fluid Flow and Transfer Processes
- Microbiology
- Ecology
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Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR. / Civilini, M.; Pucillo, C.; Colombatti, A.; Damante, G.; De Bertoldi, M.; Tell, G.
In: Letters in Applied Microbiology, Vol. 31, No. 4, 2000, p. 313-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR
AU - Civilini, M.
AU - Pucillo, C.
AU - Colombatti, A.
AU - Damante, G.
AU - De Bertoldi, M.
AU - Tell, G.
PY - 2000
Y1 - 2000
N2 - A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.
AB - A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.
UR - http://www.scopus.com/inward/record.url?scp=0033784844&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033784844&partnerID=8YFLogxK
U2 - 10.1046/j.1472-765X.2000.00819.x
DO - 10.1046/j.1472-765X.2000.00819.x
M3 - Article
C2 - 11068914
AN - SCOPUS:0033784844
VL - 31
SP - 313
EP - 318
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 4
ER -