Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

M. Civilini; C. Pucillo; A. Colombatti; G. Damante; M. De Bertoldi; G. Tell

doi:10.1046/j.1472-765X.2000.00819.x

Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

M. Civilini, C. Pucillo, A. Colombatti, G. Damante, M. De Bertoldi, G. Tell

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 10⁴ colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.

Original language	English
Pages (from-to)	313-318
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	31
Issue number	4
DOIs	https://doi.org/10.1046/j.1472-765X.2000.00819.x
Publication status	Published - 2000

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Water Science and Technology
Fluid Flow and Transfer Processes
Microbiology
Ecology

Access to Document

10.1046/j.1472-765X.2000.00819.x

Cite this

@article{d6103974a92845bf9fdb03b28f77433b,

title = "Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR",

abstract = "A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.",

author = "M. Civilini and C. Pucillo and A. Colombatti and G. Damante and {De Bertoldi}, M. and G. Tell",

year = "2000",

doi = "10.1046/j.1472-765X.2000.00819.x",

language = "English",

volume = "31",

pages = "313--318",

journal = "Letters in Applied Microbiology",

issn = "0266-8254",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

AU - Civilini, M.

AU - Pucillo, C.

AU - Colombatti, A.

AU - Damante, G.

AU - De Bertoldi, M.

AU - Tell, G.

PY - 2000

Y1 - 2000

N2 - A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.

AB - A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP(NAP) α-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 104 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.

UR - http://www.scopus.com/inward/record.url?scp=0033784844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033784844&partnerID=8YFLogxK

U2 - 10.1046/j.1472-765X.2000.00819.x

DO - 10.1046/j.1472-765X.2000.00819.x

M3 - Article

C2 - 11068914

AN - SCOPUS:0033784844

VL - 31

SP - 313

EP - 318

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 4

ER -

Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this