Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly

Paolo Ghia, Giuseppina Prato, Cristina Scielzo, Stefania Stella, Massimo Geuna, Giuseppe Guida, Federico Caligaris-Cappio

Research output: Contribution to journalArticle

Abstract

The responsiveness and diversity of peripheral B-cell repertoire decreases with age, possibly because of B-cell clonal expansions, as suggested by the incidence of serum monoclonal immunoglobulins and of monoclonal chronic lymphocytic leukemia (CLL)-like B lymphocytes in clinically silent adults. We phenotyped peripheral blood cells from 500 healthy subjects older than 65 years with no history or suspicion of malignancies and no evidence of lymphocytosis. In 19 cases (3.8%) a κ/λ ratio of more than 3:1 or less than 1:3 was found: 9 were CD5+, CD19+, CD23+, CD20 low, CD79blow, slglow (classic CLL-like phenotype); 3 were CD5+, CD19+, CD23+, CD20high, CD79blow, slglow (atypical CLL-like), and 7 were CD5-, CD19+, CD20high, CD23 -, CD79bbright, FMC7+, slgbright (non-CLL-like). In 2 subjects, 2 phenotypically distinct unrelated clones were concomitantly evident. No cases were CD10+. Polymerase chain reaction (PCR) analysis demonstrated a monoclonal rearrangement of Ig H genes in 15 of 19 cases. No bcl-1 or bcl-2 rearrangements were detected. Using a gating strategy based on CD20/CD5/CD79 expression, 13 additional CLL-like B-cell clones were identified (cumulative frequency of classic CLL-like: 5.5%). Thus, phenotypically heterogeneous monoclonal B-lymphocyte expansions are common among healthy elderly individuals and are not limited to classic CLL-like clones but may have the phenotypic features of different chronic lymphoproliferative disorders, involving also CD5- B cells.

Original languageEnglish
Pages (from-to)2337-2342
Number of pages6
JournalBlood
Volume103
Issue number6
DOIs
Publication statusPublished - Mar 15 2004

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Lymphocytes
B-Cell Chronic Lymphocytic Leukemia
Blood
B-Lymphocytes
Cells
Clone cells
Clone Cells
Polymerase chain reaction
Lymphoid Leukemia
Immunoglobulins
Lymphocytosis
Immunoglobulin Genes
Lymphoproliferative Disorders
Genes
Blood Cells
Healthy Volunteers
History
Phenotype
Polymerase Chain Reaction
Incidence

ASJC Scopus subject areas

  • Hematology

Cite this

Ghia, P., Prato, G., Scielzo, C., Stella, S., Geuna, M., Guida, G., & Caligaris-Cappio, F. (2004). Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly. Blood, 103(6), 2337-2342. https://doi.org/10.1182/blood-2003-09-3277

Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly. / Ghia, Paolo; Prato, Giuseppina; Scielzo, Cristina; Stella, Stefania; Geuna, Massimo; Guida, Giuseppe; Caligaris-Cappio, Federico.

In: Blood, Vol. 103, No. 6, 15.03.2004, p. 2337-2342.

Research output: Contribution to journalArticle

Ghia, P, Prato, G, Scielzo, C, Stella, S, Geuna, M, Guida, G & Caligaris-Cappio, F 2004, 'Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly', Blood, vol. 103, no. 6, pp. 2337-2342. https://doi.org/10.1182/blood-2003-09-3277
Ghia, Paolo ; Prato, Giuseppina ; Scielzo, Cristina ; Stella, Stefania ; Geuna, Massimo ; Guida, Giuseppe ; Caligaris-Cappio, Federico. / Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly. In: Blood. 2004 ; Vol. 103, No. 6. pp. 2337-2342.
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