Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes

Sophie Clément, Cristiana Juge-Aubry, Antonino Sgroi, Stéphanie Conzelmann, Valerio Pazienza, Brigitte Pittet-Cuenod, Christoph A. Meier, Francesco Negro

Research output: Contribution to journalArticle

Abstract

For many years, adipose tissue has been mainly considered as an inert reservoir for storing triglycerides. Since the discovery that adipocytes may secrete a variety of bioactive molecules (hormones, chemokines, and cytokines), an endocrine and paracrine role for white adipose tissue (WAT) in the regulation of energy balance and other physiological processes has been established, particularly with regard to brain and muscle. In contrast, little is known about the interactions of WAT with liver. Hence, we examined the effect of the secretory products of WAT on hepatocytes. Conditioned medium of human WAT explants induced significant steatosis in hepatocyte cell lines. Factor(s) responsible for the conditioned medium-induced steatosis were screened by a battery of blocking antibodies against different cytokines/chemokines shown to be secreted by WAT. In contrast to interleukin-8 and interleukin-6, the monocyte chemoattractant protein-1 was capable of inducing steatosis in hepatocytes in a time-dependent manner at concentrations similar to those found in conditioned medium. Incubation of conditioned medium with antimonocyte chemoattractant protein-1 antibodies prevented triglyceride accumulation. Investigation of the mechanism leading to the triglyceride accumulation showed that both a diminution of apolipoprotein B secretion and an increase in phosphoenolpyruvate carboxykinase messenger RNA may be involved. Conclusion: The monocyte chemoattractant protein-1 secreted by adipose tissue may induce steatosis not only recruiting macrophages but also acting directly on hepatocytes.

Original languageEnglish
Pages (from-to)799-807
Number of pages9
JournalHepatology
Volume48
Issue number3
DOIs
Publication statusPublished - Sep 2008

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White Adipose Tissue
Chemokine CCL2
Adipose Tissue
Hepatocytes
Conditioned Culture Medium
Lipids
Triglycerides
Chemokines
Physiological Phenomena
Cytokines
Phosphoenolpyruvate
Blocking Antibodies
Chemotactic Factors
Apolipoproteins B
Interleukin-8
Adipocytes
Interleukin-6
Macrophages
Hormones
Cell Line

ASJC Scopus subject areas

  • Hepatology

Cite this

Clément, S., Juge-Aubry, C., Sgroi, A., Conzelmann, S., Pazienza, V., Pittet-Cuenod, B., ... Negro, F. (2008). Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes. Hepatology, 48(3), 799-807. https://doi.org/10.1002/hep.22404

Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes. / Clément, Sophie; Juge-Aubry, Cristiana; Sgroi, Antonino; Conzelmann, Stéphanie; Pazienza, Valerio; Pittet-Cuenod, Brigitte; Meier, Christoph A.; Negro, Francesco.

In: Hepatology, Vol. 48, No. 3, 09.2008, p. 799-807.

Research output: Contribution to journalArticle

Clément, S, Juge-Aubry, C, Sgroi, A, Conzelmann, S, Pazienza, V, Pittet-Cuenod, B, Meier, CA & Negro, F 2008, 'Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes', Hepatology, vol. 48, no. 3, pp. 799-807. https://doi.org/10.1002/hep.22404
Clément, Sophie ; Juge-Aubry, Cristiana ; Sgroi, Antonino ; Conzelmann, Stéphanie ; Pazienza, Valerio ; Pittet-Cuenod, Brigitte ; Meier, Christoph A. ; Negro, Francesco. / Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes. In: Hepatology. 2008 ; Vol. 48, No. 3. pp. 799-807.
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