Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumor necrosis factor

Antonio Sica, Ji Ming Wang, Francesco Colotta, Elisabetta Dejana, Alberto Mantovani, Joost J. Oppenheim, Christian G. Larsen, Claus O C Zachariae, Kouji Matsushima

Research output: Contribution to journalArticle

Abstract

Inflammation, thrombosis, and immunity involve close interactions between leukocytes and vascular endothelium. Endothelial cells represent both targets and producers of lymphokines. Our study was designed to define the capacity of human endothelial cells (HEC) to produce a novel, recently purified, and molecularly cloned monocyte chemotactic and activating factor. This factor has been identified in the culture supernatants of tumor cell lines (tumor-derived chemotactic factor (TDCF)) as well as activated monocytes and fibroblasts (monocyte chemotactic and activating protein, MCAF, or monocyte chemoattractant protein-1, MCP-1). IL-1 induced high levels of production of chemotactic activity for monocytes in culture supernatants of HEC. IL-1-treated HEC expressed high levels of MCAF/MCP-1/ TDCF mRNA transcripts, as assessed by Northern blot analysis. TNF and LPS, unlike IL-6, also induced MCAF/MCP-1/TDCF gene expression. Nuclear run off experiments revealed that IL-1-activated transcription of the MCAF/MCP-1/TDCF gene. The production of MCAF/MCP-1/TDCF may represent one of the mechanisms whereby endothehal cells, exposed to inflammatory signals, participate in the regulation of leukocyte extravasation. Production of this cytokine by vascular cells may in particular be relevant under conditions of selective extravasation and activation of mononuclear phagocytes.

Original languageEnglish
Pages (from-to)3034-3038
Number of pages5
JournalJournal of Immunology
Volume144
Issue number8
Publication statusPublished - Apr 15 1990

ASJC Scopus subject areas

  • Immunology

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    Sica, A., Wang, J. M., Colotta, F., Dejana, E., Mantovani, A., Oppenheim, J. J., Larsen, C. G., Zachariae, C. O. C., & Matsushima, K. (1990). Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumor necrosis factor. Journal of Immunology, 144(8), 3034-3038.