Monocyte-derived macrophages and myeloid cell lines as targets of HIV-1 replication and persistence

Edana Cassol, Massimo Alfano, Priscilla Biswas, Guido Poli

Research output: Contribution to journalArticlepeer-review

Abstract

HIV infection of mononuclear phagocytes (MP), mostly as tissue macrophages, is a dominant feature in the pathogenesis of HIV disease and its progression to AIDS. Although the general mechanism of infection is not dissimilar to that of CD4+ lymphocytes occurring via interaction of the viral envelope with CD4 and a chemokine receptor (usually CCR5), other features are peculiar to MP infection. Among others, the long-term persistence of productive infection, sustained by the absence of substantial cell death, and the capacity of the virions to bud and accumulate in intracellular multivescicular bodies (MVB), has conferred to MP the role of "Trojan horses" perpetuating the chronic state of infection. Because the investigation of tissue macrophages is often very difficult for both ethical and practical reasons of accessibility, most studies of in vitro infection rely upon monocyte-derived macrophages (MDM), a methodology hampered by inter-patient variability and lack of uniformity of experimental protocols. A number of cell lines, mostly Mono Mac, THP-1, U937, HL-60, and their derivative chronically infected counterparts (such as UI and OM-10.1 cell lines) have complemented the MDM system of infection providing useful information on the features of HIV replication in MP. This article describes and compares the most salient features of these different cellular models of MP infection by HIV.

Original languageEnglish
Pages (from-to)1018-1030
Number of pages13
JournalJournal of Leukocyte Biology
Volume80
Issue number5
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Cytokine
  • Differentiation
  • Latency
  • Mononuclear phagocytes

ASJC Scopus subject areas

  • Cell Biology

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