Monocytes have a short half-life span and new cells must be produced in adult life to maintain appropriate numbers of these elements. The differentiation, proliferation and maturation of monocyte precursors are thought to occur only within the bone marrow. The aim of this work was to investigate if circulating monocyte precursors are present in normal human subjects. Cells were separated by standard techniques for monocytes from samples of the peripheral blood of 10 healthy subjects. The monocyte fraction was plated in T-flasks and cultured in the presence of RPMI 1640 added with 10% PCS and \% Lglutamine, without any stimulating factor. After a week, it was possible to observe cells which expressed determinants typical of immature cells, like CD]3, CD33 and CD34. The same cellular population expresses also HLA-DR and CD14, and goes by a process of further maturation, as demonstrated by the acquisition of the ability to phagocytize zymosan particles. The possible role of autocrine factor(s) in the maturation of these precursors remains to be elucidated.
|Number of pages||1|
|Publication status||Published - 1997|
ASJC Scopus subject areas
- Cancer Research
- Cell Biology