TY - JOUR
T1 - Monocytes P2X7 purinergic receptor is modulated by glatiramer acetate in multiple sclerosis
AU - Caragnano, Mariantonietta
AU - Tortorella, Paola
AU - Bergami, Alessandra
AU - Ruggieri, Maddalena
AU - Livrea, Paolo
AU - Specchio, Luigi Maria
AU - Martino, Gianvito
AU - Trojano, Maria
AU - Furlan, Roberto
AU - Avolio, Carlo
PY - 2012/4
Y1 - 2012/4
N2 - The aim of this study is to investigate the expression of P2X7R, IL-1beta and the ATP activity modulating ecto-apyrase CD39 on peripheral blood monocytes of MS patients and to observe the possible effects of Glatiramer Acetate (GA) on such expression.Twelve RR treatment-free MS patients were selected and peripheral blood monocytes were obtained. The expression of P2X7R, IL-1beta and CD39 on monocytes was investigated by qrt-PCR. The in vitro effects of GA on the expression of monocytes stimulated with BzATP (a potent P2X7R agonist)-were evaluated. Ten healthy donors (HDs) were similarly studied. Finally, 5 MS patients were given GA therapy and the monocytes obtained before treatment, after 3 and 12. months of GA treatment were similarly investigated.No differences were found in P2X7R, IL-1beta and CD39 expression between patients and controls. In MS Bz-ATP stimulated monocytes, GA pre-conditioning clearly downregulated P2X7R (p = 0.003) but IL-1beta expression also showed a decreasing trend (p = 0.07). Conversely, CD39 showed an increasing trend (p = 0.07). Similar evidence was found in HDs. GA in vivo treatment induced a reduction in the expression that was clear for P2X7R and CD39 (p <0.05) but only not significant for IL-1beta after 12. months of treatment.Monocytes from both MS and control subjects express P2X7R, IL-1beta and CD39, and GA seems to interfere with such expression.
AB - The aim of this study is to investigate the expression of P2X7R, IL-1beta and the ATP activity modulating ecto-apyrase CD39 on peripheral blood monocytes of MS patients and to observe the possible effects of Glatiramer Acetate (GA) on such expression.Twelve RR treatment-free MS patients were selected and peripheral blood monocytes were obtained. The expression of P2X7R, IL-1beta and CD39 on monocytes was investigated by qrt-PCR. The in vitro effects of GA on the expression of monocytes stimulated with BzATP (a potent P2X7R agonist)-were evaluated. Ten healthy donors (HDs) were similarly studied. Finally, 5 MS patients were given GA therapy and the monocytes obtained before treatment, after 3 and 12. months of GA treatment were similarly investigated.No differences were found in P2X7R, IL-1beta and CD39 expression between patients and controls. In MS Bz-ATP stimulated monocytes, GA pre-conditioning clearly downregulated P2X7R (p = 0.003) but IL-1beta expression also showed a decreasing trend (p = 0.07). Conversely, CD39 showed an increasing trend (p = 0.07). Similar evidence was found in HDs. GA in vivo treatment induced a reduction in the expression that was clear for P2X7R and CD39 (p <0.05) but only not significant for IL-1beta after 12. months of treatment.Monocytes from both MS and control subjects express P2X7R, IL-1beta and CD39, and GA seems to interfere with such expression.
KW - Glatiramer acetate
KW - Monocytes
KW - Multiple sclerosis
KW - P2X7 receptor
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U2 - 10.1016/j.jneuroim.2012.02.002
DO - 10.1016/j.jneuroim.2012.02.002
M3 - Article
C2 - 22370183
AN - SCOPUS:84859627499
VL - 245
SP - 93
EP - 97
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -