Morphological and biochemical features of a medroxyprogesterone acetate (MPA)-resistant MCF-7 breast cancer cell line

N. Gibelli, C. Zibera, G. Sica, M. Carbone, P. Pedrazzoli, G. R. Della Cuna

Research output: Contribution to journalArticle

Abstract

An MCF-7 human breast cancer line variant (MCF7/MPA) resistant to medroxyprogesterone-acetate (MPA) was obtained by continuous exposure in vitro to the drug. MCF-7/MPA cells were grown in the presence of 12.5 x 10-6 M MPA and were selected by increasing the concentration of the drug in the growth medium in a stepwise manner from 0.025 x 10-6 M up to 12.5 x 10-6 M. Comparative studies of cellular morphology cytosolic steroid receptor content and P-Glycoprotein expression were performed on both MCF-7 parental line and MCF-7/MPA variant. MCF-7/MPA cells when compared to the parental line exhibit a different morphology in terms of membrane alterations reduced content of cytosolic progesterone receptor increased expression of P-glycoprotein along with reduction of Doxorubicin (Dx) activity on the growth of MCF-7/MPA resistant cells.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalAnticancer Research
Volume14
Issue number1 A
Publication statusPublished - 1994

Keywords

  • Breast cancer
  • Medroxyprogesterone-acetate
  • P-glycoprotein
  • Resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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