Morphological and molecular assessment of apoptotic mechanisms in peripheral neuroblastic tumours

S. Uccini, C. Colarossi, S. Scarpino, R. Boldrini, P. G. Natali, M. R. Nicotra, F. M. Perla, O. Mannarino, P. Altavista, C. Boglino, C. A. Cappelli, D. Cozzi, A. Donfrancesco, G. Kokai, P. D. Losty, H. P. McDowell, C. Dominici

Research output: Contribution to journalArticle

Abstract

Multiple defects in apoptotic pathways have been described in peripheral neuroblastic tumours (NTs). Mitosis-karyorrhexis index (MKI) is a reliable morphological marker identifying favourable and unfavourable NTs. The extent to which apoptotic processes contribute to determine the clinical significance of MKI is still undefined. Apoptosis was investigated in a series of 110 peripheral NTs by comparing MKI to immunohistochemical and molecular apoptotic features. High MKI was found in 55 out of 110 NTs (50%) and was associated with advanced stage (P=0.007), neuroblastoma (NB) histological category (P=0.024), MYCN amplification (PL, Bax and Mcl-1 was studied by immunohistochemistry, but no significant associations were found with clinicohistological features. Microarray analysis of apoptotic genes was performed in 40 out of 110 representative tumours. No significant association was found between the expression of apoptotic genes and MKI or clinicohistological features. Proliferative activity was assessed in 60 out of 110 representative tumours using Ki67 immunostaining, but no significant correlations with MKI or clinicobiological features were found. In NTs, the combination of apoptosis and proliferation as expressed by MKI is a significant prognostic parameter, although neither of them is per se indicative of the clinicobiological behaviour and outcome.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalBritish Journal of Cancer
Volume95
Issue number1
DOIs
Publication statusPublished - Jul 3 2006

Keywords

  • Apoptosis
  • Caspases
  • Mitosis-karyorrhexis index (MKI)
  • Neuroblastoma
  • p53
  • Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Uccini, S., Colarossi, C., Scarpino, S., Boldrini, R., Natali, P. G., Nicotra, M. R., Perla, F. M., Mannarino, O., Altavista, P., Boglino, C., Cappelli, C. A., Cozzi, D., Donfrancesco, A., Kokai, G., Losty, P. D., McDowell, H. P., & Dominici, C. (2006). Morphological and molecular assessment of apoptotic mechanisms in peripheral neuroblastic tumours. British Journal of Cancer, 95(1), 49-55. https://doi.org/10.1038/sj.bjc.6603212