Morphological and ultrastructural analysis of normal, injured and osteoarthritic human knee menisci

Michela Battistelli, Marta Favero, Debora Burini, Giovanni Trisolino, Dante Dallari, Lucia De Franceschi, Steven R Goldring, Mary B Goldring, Elisa Belluzzi, Giuseppe Filardo, Brunella Grigolo, Elisabetta Falcieri, Eleonora Olivotto

Research output: Contribution to journalArticle

Abstract

The human meniscus plays a crucial role for transmission and distribution of load across the knee, as well as shock absorption, joint stability, lubrication, and congruity. The aim of this study was to compare the complex geometry, and unique ultrastructure and tissue composition of the meniscus in healthy (control) and pathological conditions to provide understanding of structural changes that could be helpful in the future design of targetted therapies and improvement of treatment indications. We analyzed meniscus samples collected from 3 healthy multi-organ donors (median age, 66 years), 5 patients with traumatic meniscal tear (median age, 41 years) and 3 patients undergoing total knee replacement (TKR) for end-stage osteoarthritis (OA) (median age, 72 years). We evaluated the extracellular matrix (ECM) organization, the appearance and distribution of areas of calcification, and modifications of cellular organization and structure by electron microscopy and histology. The ECM structure was similar in specimens from traumatic meniscus tears compared to those from patients with late-stage OA, showing disorganization of collagen fibers and increased proteoglycan content. Cells of healthy menisci showed mainly diffuse chromatin and well preserved organelles. Both in traumatic and in OA menisci, we observed increased chromatin condensation, organelle degeneration, and cytoplasmic vacuolization, a portion of which contained markers of autophagic vacuoles. Areas of calcification were also observed in both traumatic and OA menisci, as well as apoptotic-like features that were particularly prominent in traumatic meniscal tear samples. We conclude that meniscal tissue from patients with traumatic meniscal injury demonstrate pathological alterations characteristic of tissue from older patients undergoing TKR, suggesting that they have high susceptibility to develop OA.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalEuropean journal of histochemistry : EJH
Volume63
Issue number1
DOIs
Publication statusPublished - Feb 11 2019

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Knee
Osteoarthritis
Knee Replacement Arthroplasties
Tears
Organelles
Chromatin
Extracellular Matrix
Lubrication
Proteoglycans
Cellular Structures
Vacuoles
Meniscus
Shock
Histology
Electron Microscopy
Collagen
Joints
Tissue Donors
Wounds and Injuries
Therapeutics

Keywords

  • Meniscus
  • meniscal tear
  • osteoarthritis
  • transmission electron microscopy
  • extracellular matrix degeneration

Cite this

Morphological and ultrastructural analysis of normal, injured and osteoarthritic human knee menisci. / Battistelli, Michela; Favero, Marta; Burini, Debora; Trisolino, Giovanni; Dallari, Dante; De Franceschi, Lucia; Goldring, Steven R; Goldring, Mary B; Belluzzi, Elisa; Filardo, Giuseppe; Grigolo, Brunella; Falcieri, Elisabetta; Olivotto, Eleonora.

In: European journal of histochemistry : EJH, Vol. 63, No. 1, 11.02.2019, p. 17-23.

Research output: Contribution to journalArticle

Battistelli, M, Favero, M, Burini, D, Trisolino, G, Dallari, D, De Franceschi, L, Goldring, SR, Goldring, MB, Belluzzi, E, Filardo, G, Grigolo, B, Falcieri, E & Olivotto, E 2019, 'Morphological and ultrastructural analysis of normal, injured and osteoarthritic human knee menisci', European journal of histochemistry : EJH, vol. 63, no. 1, pp. 17-23. https://doi.org/10.4081/ejh.2019.2998
Battistelli, Michela ; Favero, Marta ; Burini, Debora ; Trisolino, Giovanni ; Dallari, Dante ; De Franceschi, Lucia ; Goldring, Steven R ; Goldring, Mary B ; Belluzzi, Elisa ; Filardo, Giuseppe ; Grigolo, Brunella ; Falcieri, Elisabetta ; Olivotto, Eleonora. / Morphological and ultrastructural analysis of normal, injured and osteoarthritic human knee menisci. In: European journal of histochemistry : EJH. 2019 ; Vol. 63, No. 1. pp. 17-23.
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AU - De Franceschi, Lucia

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