Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo

L. Roncali, D. Virgintino, P. Coltey, M. Bertossi, M. Errede, D. Ribatti, B. Nico, L. Mancini, S. Sorino, A. Riva

Research output: Contribution to journalArticle

Abstract

Intraventricular transplants of neural tissues were performed in ovo from embryo to embryo. Fragments of the nervous wall of the optic lobe (tectum) from 14-day chick or 12-day quail embryos (donor) were inserted into the ventricle of the right optic lobe of 6-day chick or 5-day quail embryos (host). Chick-to-chick, chick-to-quail and quail-to-chick grafts were carried out. The vascularization changes occurring in the host tectum and in the grafted neural tissues were analysed under light, transmission, and scanning electron microscopes and by morphometric methods. In the host embryo tectum, the neural graft stimulates a statistically significant increment in vessel density and a vessel sprouting into the ventricle of the optic lobe. The vascular sprouts reach the transplanted tissue and establish connections with its native microvasculature. The chick-to-quail and quail-to-chick grafts, submitted to immunoreaction with a quail-specific antibody which recognizes an antigen (MB1) present on endothelial cells, indicate that re-establishment of the circulation in the graft depends upon anastomoses between host and donor vasculatures and the rapid new growth of host-derived and donor-native vessels. The presence of macrophage-like cells escorting the new-growing vessels suggests that these cells are involved in the host and donor tissue angiogenesis.

Original languageEnglish
Pages (from-to)191-203
Number of pages13
JournalAnatomy and Embryology
Volume193
Issue number3
Publication statusPublished - 1996

Fingerprint

Quail
Embryonic Structures
Transplants
Superior Colliculi
Microvessels
Heart Ventricles
Blood Vessels
Endothelial Cells
Macrophages
Tissue Donors
Electrons
Light
Antigens
Antibodies
Growth

Keywords

  • Angiogenesis
  • Chick and quail embryo
  • Neural graft
  • Optic lobe

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

Roncali, L., Virgintino, D., Coltey, P., Bertossi, M., Errede, M., Ribatti, D., ... Riva, A. (1996). Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo. Anatomy and Embryology, 193(3), 191-203.

Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo. / Roncali, L.; Virgintino, D.; Coltey, P.; Bertossi, M.; Errede, M.; Ribatti, D.; Nico, B.; Mancini, L.; Sorino, S.; Riva, A.

In: Anatomy and Embryology, Vol. 193, No. 3, 1996, p. 191-203.

Research output: Contribution to journalArticle

Roncali, L, Virgintino, D, Coltey, P, Bertossi, M, Errede, M, Ribatti, D, Nico, B, Mancini, L, Sorino, S & Riva, A 1996, 'Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo', Anatomy and Embryology, vol. 193, no. 3, pp. 191-203.
Roncali L, Virgintino D, Coltey P, Bertossi M, Errede M, Ribatti D et al. Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo. Anatomy and Embryology. 1996;193(3):191-203.
Roncali, L. ; Virgintino, D. ; Coltey, P. ; Bertossi, M. ; Errede, M. ; Ribatti, D. ; Nico, B. ; Mancini, L. ; Sorino, S. ; Riva, A. / Morphological aspects of the vascularization in intraventricular neural transplants from embryo to embryo. In: Anatomy and Embryology. 1996 ; Vol. 193, No. 3. pp. 191-203.
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