Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg

A. Tinari, F. M. Ruggeri, M. Divizia, A. Panà, G. Donelli

Research output: Contribution to journalArticle

Abstract

Electron and immunoelectron microscopic studies were carried out on HAV-infected Frp/3 cells. The infection led to a distinctive cytopathic effect (CPE) arising on day 3 up to the complete detachment of monolayers on day 7. Infected cells exhibited progressive modifications, beginning from the formation of long helical polyribosomes. Subsequently, hypertrophy, cisternal dilatation and degranulation of the RER could be observed. Furthermore, the formation of concentric membranous bodies (CMB), large myelin-like structures and annulate lamellae could be revealed at later times of infection. 24-27 nm virus-like particles were observed within cytoplasmic vesicles or outside extensively degenerated cells. Indirect immunoperoxidase staining were used to localize HAV antigen (HAAg) in thin sectioned infected Frp/3 cells. Vesicular inclusion bodies, often seen to contain electron-lucent particles, resulted darkly stained as well as tracts of the RER and myelin-like structures. Negatively stained preparations from cell lysates revealed small clusters of HAV particles which sometimes appeared to be still associated with residual membrane fragments. Our findings seem to suggest that HAV replication occurs in close association with cytoplasmic membranes and a direct involvement of the RER seems to be demonstrated.

Original languageEnglish
Pages (from-to)209-224
Number of pages16
JournalArchives of Virology
Volume104
Issue number3-4
DOIs
Publication statusPublished - Sep 1989

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Antigens
Myelin Sheath
Cytoplasmic Vesicles
Electrons
Polyribosomes
Inclusion Bodies
Infection
Virion
Hypertrophy
Dilatation
Cell Membrane
Staining and Labeling
Membranes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Tinari, A., Ruggeri, F. M., Divizia, M., Panà, A., & Donelli, G. (1989). Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg. Archives of Virology, 104(3-4), 209-224. https://doi.org/10.1007/BF01315544

Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg. / Tinari, A.; Ruggeri, F. M.; Divizia, M.; Panà, A.; Donelli, G.

In: Archives of Virology, Vol. 104, No. 3-4, 09.1989, p. 209-224.

Research output: Contribution to journalArticle

Tinari, A, Ruggeri, FM, Divizia, M, Panà, A & Donelli, G 1989, 'Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg', Archives of Virology, vol. 104, no. 3-4, pp. 209-224. https://doi.org/10.1007/BF01315544
Tinari, A. ; Ruggeri, F. M. ; Divizia, M. ; Panà, A. ; Donelli, G. / Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg. In: Archives of Virology. 1989 ; Vol. 104, No. 3-4. pp. 209-224.
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