Abstract
Pulmonary endothelial cells are known to be fundamental for lung preservation and one of the most serious limiting factors observed during transplantation is the stress to which these cells are subjected. On this premise, strenuous efforts should be made to select and employ the preservation solution best able to prolong ischemia time and thus prevent cytotoxic effects. The aim of this study was to identify the solution with minimum toxicity on endothelial cells. For this purpose, we analysed the noxious effect of solutions such as Euro Collins (ECS), Belzer (UWS) and Low-potassium Dextran (LPD) on endothelial cells after 12 hours of incubation at 10°C. For each solution, we examined the modifications produced on the nuclei, mitochondria and cellular wall of human pulmonary-artery endothelial cells by transmission electron microscopy and recorded the results on an ultrastructural grading scale. As regards morphological alterations incompatible with cell life, the most cytotoxic solution proved to be ECS. UWS and LPD, on the contrary, appeared to preserve cells relatively well, and no perceptible difference was observed between the two solutions. In conclusion, it is interesting to note that although ECS is widely used for lung preservation, the results of our study indicate that a 12 hours at 10°C in this solution may exert adverse effect on pulmonary endothelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 61-68 |
| Number of pages | 8 |
| Journal | Journal of Medical Investigation |
| Volume | 43 |
| Issue number | 1-2 |
| Publication status | Published - Jun 1996 |
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Keywords
- Hypothermic storage
- Pulmonary-artery endothelial cells
- Toxicity of preservation solutions
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Morphological changes of human pulmonary-artery endothelial cells after 12-hours hypothermic storage in organ-preservation solutions. / Spaggiari, Lorenzo; Carbognani, Paolo; Rusca, Michele; Cattelani, Leonardo; Tecchio, Tiziano; Salcuni, Pier Franco; Bobbio, Paolo.
In: Journal of Medical Investigation, Vol. 43, No. 1-2, 06.1996, p. 61-68.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphological changes of human pulmonary-artery endothelial cells after 12-hours hypothermic storage in organ-preservation solutions
AU - Spaggiari, Lorenzo
AU - Carbognani, Paolo
AU - Rusca, Michele
AU - Cattelani, Leonardo
AU - Tecchio, Tiziano
AU - Salcuni, Pier Franco
AU - Bobbio, Paolo
PY - 1996/6
Y1 - 1996/6
N2 - Pulmonary endothelial cells are known to be fundamental for lung preservation and one of the most serious limiting factors observed during transplantation is the stress to which these cells are subjected. On this premise, strenuous efforts should be made to select and employ the preservation solution best able to prolong ischemia time and thus prevent cytotoxic effects. The aim of this study was to identify the solution with minimum toxicity on endothelial cells. For this purpose, we analysed the noxious effect of solutions such as Euro Collins (ECS), Belzer (UWS) and Low-potassium Dextran (LPD) on endothelial cells after 12 hours of incubation at 10°C. For each solution, we examined the modifications produced on the nuclei, mitochondria and cellular wall of human pulmonary-artery endothelial cells by transmission electron microscopy and recorded the results on an ultrastructural grading scale. As regards morphological alterations incompatible with cell life, the most cytotoxic solution proved to be ECS. UWS and LPD, on the contrary, appeared to preserve cells relatively well, and no perceptible difference was observed between the two solutions. In conclusion, it is interesting to note that although ECS is widely used for lung preservation, the results of our study indicate that a 12 hours at 10°C in this solution may exert adverse effect on pulmonary endothelial cells.
AB - Pulmonary endothelial cells are known to be fundamental for lung preservation and one of the most serious limiting factors observed during transplantation is the stress to which these cells are subjected. On this premise, strenuous efforts should be made to select and employ the preservation solution best able to prolong ischemia time and thus prevent cytotoxic effects. The aim of this study was to identify the solution with minimum toxicity on endothelial cells. For this purpose, we analysed the noxious effect of solutions such as Euro Collins (ECS), Belzer (UWS) and Low-potassium Dextran (LPD) on endothelial cells after 12 hours of incubation at 10°C. For each solution, we examined the modifications produced on the nuclei, mitochondria and cellular wall of human pulmonary-artery endothelial cells by transmission electron microscopy and recorded the results on an ultrastructural grading scale. As regards morphological alterations incompatible with cell life, the most cytotoxic solution proved to be ECS. UWS and LPD, on the contrary, appeared to preserve cells relatively well, and no perceptible difference was observed between the two solutions. In conclusion, it is interesting to note that although ECS is widely used for lung preservation, the results of our study indicate that a 12 hours at 10°C in this solution may exert adverse effect on pulmonary endothelial cells.
KW - Hypothermic storage
KW - Pulmonary-artery endothelial cells
KW - Toxicity of preservation solutions
UR - http://www.scopus.com/inward/record.url?scp=14844337568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14844337568&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:14844337568
VL - 43
SP - 61
EP - 68
JO - Journal of Medical Investigation
JF - Journal of Medical Investigation
SN - 1343-1420
IS - 1-2
ER -