Morphological modifications induced by HCV infection in the TOFE human lymphoblastoid cell line

A. Serafino, M. B. Valli, F. Andreola, G. Carloni, L. Bertolini

Research output: Contribution to journalArticlepeer-review


In this study, we analysed by transmission electron microscopy (TEM), sequential details of morphological modifications that accompanied viral morphogenesis in the lymphoblastoid cell line (LCL) TOFE infected in vitro with hepatitis C virus (HCV). As previously reported, we observed virus-like particles (VLPs) in cytoplasmic vesicles mainly located in the perinuclear region of infected cells. In this area, the Golgi apparatus and the endoplasmic reticulum (ER) appeared hyperplastic, remarkably enriched in vesicles and lysosomal structures. Furthermore, only in this perinuclear region, cytopathic-effect(CPE)-like changes seemed to originate, consisting in enlarged cytoplasmic vacuoles filled with degenerative amorphous material containing VLPs. Finally, the complete filling-up of the cytoplasm with these degenerative vacuoles, in addition to cellular lysis displayed by some cells, appeared as the possible terminal pattern of the infectious process. Our data suggest that in vitro HCV-infected TOFE cells undergo typical CPE-like changes that may be connected with virus replication.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalResearch in Virology
Issue number5
Publication statusPublished - Sep 1998


  • Cytopathic-like effect
  • HCV
  • TOFE cell line
  • Ultrastructural changes

ASJC Scopus subject areas

  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Morphological modifications induced by HCV infection in the TOFE human lymphoblastoid cell line'. Together they form a unique fingerprint.

Cite this