Morphologically typical and atypical B-cell chronic lymphocytic leukemias display a different pattern of surface antigenic density

G. D'arena, M. Dell'olio, P. Musto, N. Cascavilla, G. Perla, L. Savino, M. M. Greco

Research output: Contribution to journalArticlepeer-review

Abstract

Recent evidences suggest that B-cell chronic lymphocytic leukemia (B-CLL) may have heterogeneous biological and clinical features. Immunological phenotype may be useful for distinguishing these different forms of disease. We used a quantitative flow cytometric approach to analyze the expression of several membrane molecules (CD19, CD20, CD22, CD23, CD11c, CD5, CD79b) commonly used to diagnose and characterize B-CLL in a choort of 84 consecutive B-CLL patients diagnosed according to morphological and immunological findings. We found that morphologically so-called "atypical" B-CLL displayed a significantly higher number of CD20 and CD22 molecules than typical forms. On the other hand, CD19 was found to be more expressed in typical B-CLL, although without reaching statistical significance. Finally, no difference was detected with respect to CD23, CD79b, CD11c and CD5 number of molecules/per cell between typical and atypical B-CLL. Other clinico-biological features, such as surface membrane immunoglobulin density, percentage of CD79b and FMC7 expression, peripheral blood lymphocytosis, trisomy 12 and advanced clinical stages were also found to be more frequent in atypical B-CLL. In conclusion, our data confirm the hypothesis that atypical B-CLL is a disease sustained by more mature B-cells, closely related but, at the same time, clearly distincted from neoplastic cells of typical B-CLL.

Original languageEnglish
Pages (from-to)649-654
Number of pages6
JournalLeukemia and Lymphoma
Volume42
Issue number4
Publication statusPublished - 2001

Keywords

  • Chronic lymphocytic leukemia
  • Morphology
  • Quantitative flow cytometry

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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