TY - JOUR
T1 - Morphometric analysis and topological organization of nuclear matrix in freeze-fractured electron microscopy
AU - Maraldi, Nadir M.
AU - Marinelli, Fiorenzo
AU - Cocco, Lucio
AU - Papa, Stefano
AU - Santi, Patrizia
AU - Manzoli, Francesco A.
PY - 1986
Y1 - 1986
N2 - The ultrastructural organization of nuclear matrix, purified from intact or membrane-denuded rat liver nuclei, has been analysed by means of freeze-fracturing technique. This method avoids dehydration and embedding which, in conventional thin sectioning, partly distort or mask the matrix ultrastructure. The various matrix components, and mainly the peripheral lamina and the inner network revealed complex arrangements undetectable with conventional techniques. Morphometric analyses performed with a Texture Analysis System (TAS) Leitz, allowed to obtain precise information on the matrix constituents, based on the histograms of their size distribution. These textural characteristics have been utilized in order to identify, by means of a particular computer programme, the putative matrix localization within intact freeze-fractured nuclei.
AB - The ultrastructural organization of nuclear matrix, purified from intact or membrane-denuded rat liver nuclei, has been analysed by means of freeze-fracturing technique. This method avoids dehydration and embedding which, in conventional thin sectioning, partly distort or mask the matrix ultrastructure. The various matrix components, and mainly the peripheral lamina and the inner network revealed complex arrangements undetectable with conventional techniques. Morphometric analyses performed with a Texture Analysis System (TAS) Leitz, allowed to obtain precise information on the matrix constituents, based on the histograms of their size distribution. These textural characteristics have been utilized in order to identify, by means of a particular computer programme, the putative matrix localization within intact freeze-fractured nuclei.
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U2 - 10.1016/0014-4827(86)90066-2
DO - 10.1016/0014-4827(86)90066-2
M3 - Article
C2 - 3754218
AN - SCOPUS:0022539918
VL - 163
SP - 349
EP - 362
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -