Morphometric study of chromatin pattern in freeze-fractured rat liver nuclei during malignancy evolution

F. Marinelli, S. Squarzoni, N. M. Maraldi, C. DiLoreto, C. Rubini, G. M. Mariuzzi, R. Castagnani

Research output: Contribution to journalArticlepeer-review

Abstract

Computerized image analysis was performed on freeze-fractured nuclei isolated from normal rat liver and rat liver with diethylnitrosamine induced hypatocarcinomas. Chromatin particles belonging to heterochromatin and interchromatin nuclear regions were measured, distributed into histograms and statistically processed. The ratio between nucleosome filaments (5-10 nm range diameter) and solenoid fibres (25-35 nm range) and Turkey's fitting line were calculated. The N/S ratio shows an inverse trend in treated nuclear samples with respect to the controls indicating that the malignancy progression is accompanied by characteristic derangement of the chromatin arrangement which can be evaluated by morphometric analysis.

Original languageEnglish
Pages (from-to)769-773
Number of pages5
JournalPathology Research and Practice
Volume185
Issue number5
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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