TY - JOUR
T1 - Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold
AU - Tetè, Stefano
AU - Mastrangelo, F.
AU - Carone, L.
AU - Nargi, E.
AU - Costanzo, G.
AU - Vinci, R.
AU - Burruano, F.
AU - Tortorici, S.
AU - Dadorante, V.
AU - Caciagli, F.
AU - Traini, T.
AU - Gherlone, E.
AU - Caraffa, A.
AU - Salini, V.
AU - Conti, P.
AU - Ciccarelli, R.
PY - 2007/10
Y1 - 2007/10
N2 - In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.
AB - In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.
KW - Dental follicle
KW - Porous bone hydroxyapatite (Bio-Oss®)
KW - Stem cells
KW - Tissue engineering
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UR - http://www.scopus.com/inward/citedby.url?scp=37749055419&partnerID=8YFLogxK
M3 - Article
C2 - 18179737
AN - SCOPUS:37749055419
VL - 20
SP - 819
EP - 826
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
IS - 4
ER -