Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia

Luigi Maiuri, Valeria Raia, Jenny Potter, Dallas Swallow, Mae Wan Ho, Roberto Fiocca, Giovanna Finzi, Matteo Cornaggia, Carlo Capella, Andrea Quaroni, Salvatore Auricchio

Research output: Contribution to journalArticle

Abstract

Immunohistological analysis of the expression of lactase protein in adults with hypolactasia has been carried out using monoclonal antibodies. Eight different antibodies that recognize at least three distinct epitopes on the lactase protein each gave the same result. Strong brush border staining was observed in all the lactase-persistent adults. No staining at all was detected in 9 of the hypolactasic subjects. In the remaining 12 individuals a mosaic pattern of expression was observed: small patches of enterocytes stained strongly, whereas the surrounding areas showed no staining at all. Sucraseisomaltase, in contrast, showed no such mosaicism in these or in any of the other individuals. The mosaicism observed in the 12 hypolactasic individuals suggests that the differentiation of the columnar cells along the villus is not homogeneous. Furthermore, the existence of two patterns of expression of the lactase protein in the lactase-deficient individuals (i.e., absence of protein and mosaicism), if characteristic of the entire length of the intestine of the individuals tested, would suggest the existence of two phenotypes of adult-type hypolactasia in the population studied.

Original languageEnglish
Pages (from-to)359-369
Number of pages11
JournalGastroenterology
Volume100
Issue number2
Publication statusPublished - 1991

Fingerprint

Lactase
Enterocytes
Mosaicism
Staining and Labeling
Proteins
Lactose Intolerance
Microvilli
Intestines
Epitopes
Cell Differentiation
Monoclonal Antibodies
Adult Type Lactose Intolerance
Phenotype
Antibodies
Population

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Maiuri, L., Raia, V., Potter, J., Swallow, D., Ho, M. W., Fiocca, R., ... Auricchio, S. (1991). Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia. Gastroenterology, 100(2), 359-369.

Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia. / Maiuri, Luigi; Raia, Valeria; Potter, Jenny; Swallow, Dallas; Ho, Mae Wan; Fiocca, Roberto; Finzi, Giovanna; Cornaggia, Matteo; Capella, Carlo; Quaroni, Andrea; Auricchio, Salvatore.

In: Gastroenterology, Vol. 100, No. 2, 1991, p. 359-369.

Research output: Contribution to journalArticle

Maiuri, L, Raia, V, Potter, J, Swallow, D, Ho, MW, Fiocca, R, Finzi, G, Cornaggia, M, Capella, C, Quaroni, A & Auricchio, S 1991, 'Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia', Gastroenterology, vol. 100, no. 2, pp. 359-369.
Maiuri, Luigi ; Raia, Valeria ; Potter, Jenny ; Swallow, Dallas ; Ho, Mae Wan ; Fiocca, Roberto ; Finzi, Giovanna ; Cornaggia, Matteo ; Capella, Carlo ; Quaroni, Andrea ; Auricchio, Salvatore. / Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia. In: Gastroenterology. 1991 ; Vol. 100, No. 2. pp. 359-369.
@article{45e6346fa29b49629bc364c310e074b6,
title = "Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia",
abstract = "Immunohistological analysis of the expression of lactase protein in adults with hypolactasia has been carried out using monoclonal antibodies. Eight different antibodies that recognize at least three distinct epitopes on the lactase protein each gave the same result. Strong brush border staining was observed in all the lactase-persistent adults. No staining at all was detected in 9 of the hypolactasic subjects. In the remaining 12 individuals a mosaic pattern of expression was observed: small patches of enterocytes stained strongly, whereas the surrounding areas showed no staining at all. Sucraseisomaltase, in contrast, showed no such mosaicism in these or in any of the other individuals. The mosaicism observed in the 12 hypolactasic individuals suggests that the differentiation of the columnar cells along the villus is not homogeneous. Furthermore, the existence of two patterns of expression of the lactase protein in the lactase-deficient individuals (i.e., absence of protein and mosaicism), if characteristic of the entire length of the intestine of the individuals tested, would suggest the existence of two phenotypes of adult-type hypolactasia in the population studied.",
author = "Luigi Maiuri and Valeria Raia and Jenny Potter and Dallas Swallow and Ho, {Mae Wan} and Roberto Fiocca and Giovanna Finzi and Matteo Cornaggia and Carlo Capella and Andrea Quaroni and Salvatore Auricchio",
year = "1991",
language = "English",
volume = "100",
pages = "359--369",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "2",

}

TY - JOUR

T1 - Mosaic pattern of lactase expression by villous enterocytes in human adult-type hypolactasia

AU - Maiuri, Luigi

AU - Raia, Valeria

AU - Potter, Jenny

AU - Swallow, Dallas

AU - Ho, Mae Wan

AU - Fiocca, Roberto

AU - Finzi, Giovanna

AU - Cornaggia, Matteo

AU - Capella, Carlo

AU - Quaroni, Andrea

AU - Auricchio, Salvatore

PY - 1991

Y1 - 1991

N2 - Immunohistological analysis of the expression of lactase protein in adults with hypolactasia has been carried out using monoclonal antibodies. Eight different antibodies that recognize at least three distinct epitopes on the lactase protein each gave the same result. Strong brush border staining was observed in all the lactase-persistent adults. No staining at all was detected in 9 of the hypolactasic subjects. In the remaining 12 individuals a mosaic pattern of expression was observed: small patches of enterocytes stained strongly, whereas the surrounding areas showed no staining at all. Sucraseisomaltase, in contrast, showed no such mosaicism in these or in any of the other individuals. The mosaicism observed in the 12 hypolactasic individuals suggests that the differentiation of the columnar cells along the villus is not homogeneous. Furthermore, the existence of two patterns of expression of the lactase protein in the lactase-deficient individuals (i.e., absence of protein and mosaicism), if characteristic of the entire length of the intestine of the individuals tested, would suggest the existence of two phenotypes of adult-type hypolactasia in the population studied.

AB - Immunohistological analysis of the expression of lactase protein in adults with hypolactasia has been carried out using monoclonal antibodies. Eight different antibodies that recognize at least three distinct epitopes on the lactase protein each gave the same result. Strong brush border staining was observed in all the lactase-persistent adults. No staining at all was detected in 9 of the hypolactasic subjects. In the remaining 12 individuals a mosaic pattern of expression was observed: small patches of enterocytes stained strongly, whereas the surrounding areas showed no staining at all. Sucraseisomaltase, in contrast, showed no such mosaicism in these or in any of the other individuals. The mosaicism observed in the 12 hypolactasic individuals suggests that the differentiation of the columnar cells along the villus is not homogeneous. Furthermore, the existence of two patterns of expression of the lactase protein in the lactase-deficient individuals (i.e., absence of protein and mosaicism), if characteristic of the entire length of the intestine of the individuals tested, would suggest the existence of two phenotypes of adult-type hypolactasia in the population studied.

UR - http://www.scopus.com/inward/record.url?scp=0026069545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026069545&partnerID=8YFLogxK

M3 - Article

C2 - 1702075

AN - SCOPUS:0026069545

VL - 100

SP - 359

EP - 369

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 2

ER -