Abstract
Polymerase chain reaction was performed in 251 infants born to HIV-1-seropositive mothers to diagnose HIV-1 infection. Assay specificity was invariably > 95%, regardless of age at testing, while sensitivity ranged from 15% in neonates (within 48 h of birth) to > 95% in infants over 1 month of age. Evaluation of viral burden in 43 infected infants by means of quantitative DNA-PCR disclosed that the number of HIV-1 proviruses ranged from 5 to 947 per 100,000 peripheral blood mononuclear cells. Clinical follow-up demonstrated that a high viral burden was associated significantly with disease onset.
| Original language | English |
|---|---|
| Pages (from-to) | 25-28 |
| Number of pages | 4 |
| Journal | Acta Paediatrica, International Journal of Paediatrics, Supplement |
| Volume | 83 |
| Issue number | 400 |
| Publication status | Published - 1994 |
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Keywords
- diagnosis
- PCR
- perinatal HIV-1 infection
- viral burden
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Mother-to-child HIV-1 transmission : Quantitative assessment of viral burden as a diagnostic tool and prognostic parameter in HIV-1-infected children. / De Rossi, A.; Ometto, L.; Zanotto, C.; Salvatori, F.; Masiero, S.; Mammano, F.; Chieco-Bianchi, L.
In: Acta Paediatrica, International Journal of Paediatrics, Supplement, Vol. 83, No. 400, 1994, p. 25-28.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mother-to-child HIV-1 transmission
T2 - Quantitative assessment of viral burden as a diagnostic tool and prognostic parameter in HIV-1-infected children
AU - De Rossi, A.
AU - Ometto, L.
AU - Zanotto, C.
AU - Salvatori, F.
AU - Masiero, S.
AU - Mammano, F.
AU - Chieco-Bianchi, L.
PY - 1994
Y1 - 1994
N2 - Polymerase chain reaction was performed in 251 infants born to HIV-1-seropositive mothers to diagnose HIV-1 infection. Assay specificity was invariably > 95%, regardless of age at testing, while sensitivity ranged from 15% in neonates (within 48 h of birth) to > 95% in infants over 1 month of age. Evaluation of viral burden in 43 infected infants by means of quantitative DNA-PCR disclosed that the number of HIV-1 proviruses ranged from 5 to 947 per 100,000 peripheral blood mononuclear cells. Clinical follow-up demonstrated that a high viral burden was associated significantly with disease onset.
AB - Polymerase chain reaction was performed in 251 infants born to HIV-1-seropositive mothers to diagnose HIV-1 infection. Assay specificity was invariably > 95%, regardless of age at testing, while sensitivity ranged from 15% in neonates (within 48 h of birth) to > 95% in infants over 1 month of age. Evaluation of viral burden in 43 infected infants by means of quantitative DNA-PCR disclosed that the number of HIV-1 proviruses ranged from 5 to 947 per 100,000 peripheral blood mononuclear cells. Clinical follow-up demonstrated that a high viral burden was associated significantly with disease onset.
KW - diagnosis
KW - PCR
KW - perinatal HIV-1 infection
KW - viral burden
UR - http://www.scopus.com/inward/record.url?scp=0028142305&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028142305&partnerID=8YFLogxK
M3 - Article
VL - 83
SP - 25
EP - 28
JO - Acta Paediatrica, International Journal of Paediatrics, Supplement
JF - Acta Paediatrica, International Journal of Paediatrics, Supplement
SN - 0803-5326
IS - 400
ER -