Mother-to-child HIV-1 transmission

Quantitative assessment of viral burden as a diagnostic tool and prognostic parameter in HIV-1-infected children

A. De Rossi, L. Ometto, C. Zanotto, F. Salvatori, S. Masiero, F. Mammano, L. Chieco-Bianchi

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction was performed in 251 infants born to HIV-1-seropositive mothers to diagnose HIV-1 infection. Assay specificity was invariably > 95%, regardless of age at testing, while sensitivity ranged from 15% in neonates (within 48 h of birth) to > 95% in infants over 1 month of age. Evaluation of viral burden in 43 infected infants by means of quantitative DNA-PCR disclosed that the number of HIV-1 proviruses ranged from 5 to 947 per 100,000 peripheral blood mononuclear cells. Clinical follow-up demonstrated that a high viral burden was associated significantly with disease onset.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalActa Paediatrica, International Journal of Paediatrics, Supplement
Volume83
Issue number400
Publication statusPublished - 1994

Fingerprint

Viral Load
HIV-1
Mothers
Proviruses
Polymerase Chain Reaction
HIV Infections
Blood Cells
Parturition
Newborn Infant
DNA

Keywords

  • diagnosis
  • PCR
  • perinatal HIV-1 infection
  • viral burden

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Mother-to-child HIV-1 transmission : Quantitative assessment of viral burden as a diagnostic tool and prognostic parameter in HIV-1-infected children. / De Rossi, A.; Ometto, L.; Zanotto, C.; Salvatori, F.; Masiero, S.; Mammano, F.; Chieco-Bianchi, L.

In: Acta Paediatrica, International Journal of Paediatrics, Supplement, Vol. 83, No. 400, 1994, p. 25-28.

Research output: Contribution to journalArticle

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AU - Chieco-Bianchi, L.

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