Mouse hepatocytes lacking mGlu5 metabotropic glutamate receptors are less sensitive to hypoxic damage

Marianna Storto; Giuseppe Battaglia; Roberto Gradini; Valeria Bruno; Ferdinando Nicoletti; Mariapia Vairetti

doi:10.1016/j.ejphar.2004.06.023

Mouse hepatocytes lacking mGlu5 metabotropic glutamate receptors are less sensitive to hypoxic damage

Marianna Storto, Giuseppe Battaglia, Roberto Gradini, Valeria Bruno, Ferdinando Nicoletti, Mariapia Vairetti

www.neuromed.it

Research output: Contribution to journal › Article › peer-review

Abstract

Endogenous activation of type-5 metabotropic glutamate receptors contributes to the development of hypoxia-induced liver cell injury. We have strengthened this hypothesis using glutamate mGlu5 receptor knockout mice. Hepatocytes isolated from knockout mice were less sensitive to hypoxic cell damage than hepatocytes from wild-type mice as assessed by lactate dehydrogenase release and formation of reactive oxygen species. The mGlu5 receptor antagonist, 2-methyl-6-(phenylethynyl)pyridine (MPEP) also protect hepatocytes against hypoxic damage.

Original language	English
Pages (from-to)	25-27
Number of pages	3
Journal	European Journal of Pharmacology
Volume	497
Issue number	1
DOIs	https://doi.org/10.1016/j.ejphar.2004.06.023
Publication status	Published - Aug 16 2004

Keywords

Hypoxia
mGlu5 knockout
mouse
Reactive oxygen species

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Pharmacology

Access to Document

10.1016/j.ejphar.2004.06.023

Fingerprint Dive into the research topics of 'Mouse hepatocytes lacking mGlu5 metabotropic glutamate receptors are less sensitive to hypoxic damage'. Together they form a unique fingerprint.

Cite this

@article{ea2e573eaf8e442a93dbbc56ccf97efa,

title = "Mouse hepatocytes lacking mGlu5 metabotropic glutamate receptors are less sensitive to hypoxic damage",

abstract = "Endogenous activation of type-5 metabotropic glutamate receptors contributes to the development of hypoxia-induced liver cell injury. We have strengthened this hypothesis using glutamate mGlu5 receptor knockout mice. Hepatocytes isolated from knockout mice were less sensitive to hypoxic cell damage than hepatocytes from wild-type mice as assessed by lactate dehydrogenase release and formation of reactive oxygen species. The mGlu5 receptor antagonist, 2-methyl-6-(phenylethynyl)pyridine (MPEP) also protect hepatocytes against hypoxic damage.",

keywords = "Hypoxia, mGlu5 knockout, mouse, Reactive oxygen species",

author = "Marianna Storto and Giuseppe Battaglia and Roberto Gradini and Valeria Bruno and Ferdinando Nicoletti and Mariapia Vairetti",

year = "2004",

month = aug,

day = "16",

doi = "10.1016/j.ejphar.2004.06.023",

language = "English",

volume = "497",

pages = "25--27",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Mouse hepatocytes lacking mGlu5 metabotropic glutamate receptors are less sensitive to hypoxic damage

AU - Storto, Marianna

AU - Battaglia, Giuseppe

AU - Gradini, Roberto

AU - Bruno, Valeria

AU - Nicoletti, Ferdinando

AU - Vairetti, Mariapia

PY - 2004/8/16

Y1 - 2004/8/16

N2 - Endogenous activation of type-5 metabotropic glutamate receptors contributes to the development of hypoxia-induced liver cell injury. We have strengthened this hypothesis using glutamate mGlu5 receptor knockout mice. Hepatocytes isolated from knockout mice were less sensitive to hypoxic cell damage than hepatocytes from wild-type mice as assessed by lactate dehydrogenase release and formation of reactive oxygen species. The mGlu5 receptor antagonist, 2-methyl-6-(phenylethynyl)pyridine (MPEP) also protect hepatocytes against hypoxic damage.

AB - Endogenous activation of type-5 metabotropic glutamate receptors contributes to the development of hypoxia-induced liver cell injury. We have strengthened this hypothesis using glutamate mGlu5 receptor knockout mice. Hepatocytes isolated from knockout mice were less sensitive to hypoxic cell damage than hepatocytes from wild-type mice as assessed by lactate dehydrogenase release and formation of reactive oxygen species. The mGlu5 receptor antagonist, 2-methyl-6-(phenylethynyl)pyridine (MPEP) also protect hepatocytes against hypoxic damage.

KW - Hypoxia

KW - mGlu5 knockout

KW - mouse

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=4143056101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143056101&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2004.06.023

DO - 10.1016/j.ejphar.2004.06.023

M3 - Article

C2 - 15321731

AN - SCOPUS:4143056101

VL - 497

SP - 25

EP - 27

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -