Mouse macrophage clones immortalized by retroviruses are functionally heterogeneous

Laura Pirami, Brigitta Stockinger, Sally Betz Corradin, Marina Sironi, Marica Sassano, Paola Valsasnini, Marco Righi, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

Abstract

Murine macrophage clones were generated from thymus, spleen, brain, and bone marrow by in vitro immortalization with recombinant retroviruses carrying an avian v-myc oncogene. The cloned cell lines express F4/80 molecules, exert phagocytosis, have nonspecific esterase activity, and express class II molecules after Interferon γ activation. The macrophage clones are diploid and their karyotypes have remained stable for >3 years in culture. After the macrophage clones were activated, their pattern of cytokine production was investigated. Functional heterogeneity in cytokine transcription was demonstrated: one of six liposaccharide-activated macrophages was unable to transcribe interleukin la, whereas all of the liposaccharide-activated clones were able to transcribe tumor necrosis factor α. Interleukin 6 production was detected in three of six clones. The production of nitrite and tumor necrosis factor α as effector molecules of cytotoxicity was detected in all clones, thus showing that a single macrophage can exert more than one cytotoxic mechanism. The results indicate that immortalized and cloned macrophages have a differentially regulated expression of cytokine genes, adding further evidence for the existence of functional heterogeneity among cloned macrophages. This heterogeneity seems to derive from differentiation-related mechanisms rather than from external constraints.

Original languageEnglish
Pages (from-to)7543-7547
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number17
Publication statusPublished - 1991

Keywords

  • Cytokine expression
  • Cytotoxicity
  • Macrophage differentiation

ASJC Scopus subject areas

  • General
  • Genetics

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  • Cite this

    Pirami, L., Stockinger, B., Corradin, S. B., Sironi, M., Sassano, M., Valsasnini, P., Righi, M., & Ricciardi-Castagnoli, P. (1991). Mouse macrophage clones immortalized by retroviruses are functionally heterogeneous. Proceedings of the National Academy of Sciences of the United States of America, 88(17), 7543-7547.