MPA increases Idarubicin-induced apoptosis in Chronic Lymphatic Leukaemia cells via Caspase-3

Salvatore Florio, Luca Crispino, Roberto Ciarcia, Giovanni Vacca, Ugo Pagnini, Andrea De Matteis, Carmen Pacilio, Giuseppina D'Andrilli, Christine Kumar, Antonio Giordano

Research output: Contribution to journalArticlepeer-review

Abstract

The caspase family of protease is speculated to have a crucial role in apoptosis. The effect of treatment with Idarubicin (IDA) and Medroxyprogesterone acetate (MPA), used alone or in combination, on the activation of Caspase-3 in canine Chronic Lymphatic Leukaemia (CLL) cells was investigated, in order to clarify the mechanism of chemo- and hormone-therapy mediated apoptosis. Caspase activity was determined by a quantitative fluorimetric assay. Apoptosis was monitored by propidium iodide (PI) and nucleosomes assay. Treatment of CLL cells for 24 h with MPA 5 μM did not significantly activate caspase-3 but its activity was increased almost 5-fold more with IDA 1 μM (P

Original languageEnglish
Pages (from-to)747-754
Number of pages8
JournalJournal of Cellular Biochemistry
Volume89
Issue number4
DOIs
Publication statusPublished - Jul 1 2003

Keywords

  • Apoptosis
  • Caspase-3
  • Idarubicin
  • Medroxyprogesterone acetate

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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