MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase

Sara Vidoni, Michael E. Harbour, Sergio Guerrero-Castillo, Alba Signes, Shujing Ding, Ian M. Fearnley, Robert W. Taylor, Valeria Tiranti, Susanne Arnold, Erika Fernandez-Vizarra, Massimo Zeviani

Research output: Contribution to journalArticle

Abstract

© 2017 Elsevier Inc. The biogenesis of human cytochrome c oxidase (COX) is an intricate process in which three mitochondrial DNA (mtDNA)-encoded core subunits are assembled in a coordinated way with at least 11 nucleus-encoded subunits. Many chaperones shared between yeast and humans are involved in COX assembly. Here, we have used a MT-CO3 mutant cybrid cell line to define the composition of assembly intermediates and identify new human COX assembly factors. Quantitative mass spectrometry analysis led us to modify the assembly model from a sequential pathway to a module-based process. Each module contains one of the three core subunits, together with different ancillary components, includin g HIGD1A. By the same analysis, we identified the short isoform of the myofibrillogenesis regulator 1 (MR-1S) as a new COX assembly factor, which works with the highly conserved PET100 and PET117 chaperones to assist COX biogenesis in higher eukaryotes.
Original languageEnglish
Pages (from-to)1727-1738
Number of pages12
JournalCell Reports
Volume18
Issue number7
DOIs
Publication statusPublished - Feb 14 2017

Keywords

  • complexome profiling
  • COX assembly
  • cytochrome c oxidase
  • mitochondrial respiratory chain
  • MR-1S
  • myofibrillogenesis regulator 1 short isoform
  • PET100
  • PET117
  • SILAC

Fingerprint Dive into the research topics of 'MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase'. Together they form a unique fingerprint.

  • Cite this

    Vidoni, S., Harbour, M. E., Guerrero-Castillo, S., Signes, A., Ding, S., Fearnley, I. M., Taylor, R. W., Tiranti, V., Arnold, S., Fernandez-Vizarra, E., & Zeviani, M. (2017). MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase. Cell Reports, 18(7), 1727-1738. https://doi.org/10.1016/j.celrep.2017.01.044