Abstract
The expression of the N-methyl-D-aspartate (NMDA) receptor subunit NR2B/ε2 (GRIN2B) in the human adult brain was assayed by in situ hybridisation, by using a specific cRNA probe. The full length GRIN2B cDNA was cloned and sequenced. It showed a 90% nucleotide conservation when compared to the rodent homologue. GRIN2B gene is expressed at high levels in the fronto-parieto-temporal cortex and hippocampus pyramidal cells and, at a lower extent, in the basal ganglia (amygdala and striatum). The cerebellar granule cells does not show any mRNA expression. The non-ubiquitous anatomical distribution of the GRIN2B mRNA in the central nervous system suggests that the gene could be involved in specific functions pertaining to the expressing cell groups.
| Original language | English |
|---|---|
| Pages (from-to) | 49-53 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 239 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 12 1997 |
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Keywords
- Glutamate
- GRIN2B
- In situ hybridisation
- N-Methyl-D-aspartate
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
mRNA distribution in adult human brain of GRIN2B, a N-methyl-D-aspartate (NMDA) receptor subunit. / Schito, A. M.; Pizzuti, A.; Di Maria, E.; Schenone, A.; Ratti, A.; Defferrari, R.; Bellone, E.; Mancardi, G. L.; Ajmar, F.; Mandich, P.
In: Neuroscience Letters, Vol. 239, No. 1, 12.12.1997, p. 49-53.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - mRNA distribution in adult human brain of GRIN2B, a N-methyl-D-aspartate (NMDA) receptor subunit
AU - Schito, A. M.
AU - Pizzuti, A.
AU - Di Maria, E.
AU - Schenone, A.
AU - Ratti, A.
AU - Defferrari, R.
AU - Bellone, E.
AU - Mancardi, G. L.
AU - Ajmar, F.
AU - Mandich, P.
PY - 1997/12/12
Y1 - 1997/12/12
N2 - The expression of the N-methyl-D-aspartate (NMDA) receptor subunit NR2B/ε2 (GRIN2B) in the human adult brain was assayed by in situ hybridisation, by using a specific cRNA probe. The full length GRIN2B cDNA was cloned and sequenced. It showed a 90% nucleotide conservation when compared to the rodent homologue. GRIN2B gene is expressed at high levels in the fronto-parieto-temporal cortex and hippocampus pyramidal cells and, at a lower extent, in the basal ganglia (amygdala and striatum). The cerebellar granule cells does not show any mRNA expression. The non-ubiquitous anatomical distribution of the GRIN2B mRNA in the central nervous system suggests that the gene could be involved in specific functions pertaining to the expressing cell groups.
AB - The expression of the N-methyl-D-aspartate (NMDA) receptor subunit NR2B/ε2 (GRIN2B) in the human adult brain was assayed by in situ hybridisation, by using a specific cRNA probe. The full length GRIN2B cDNA was cloned and sequenced. It showed a 90% nucleotide conservation when compared to the rodent homologue. GRIN2B gene is expressed at high levels in the fronto-parieto-temporal cortex and hippocampus pyramidal cells and, at a lower extent, in the basal ganglia (amygdala and striatum). The cerebellar granule cells does not show any mRNA expression. The non-ubiquitous anatomical distribution of the GRIN2B mRNA in the central nervous system suggests that the gene could be involved in specific functions pertaining to the expressing cell groups.
KW - Glutamate
KW - GRIN2B
KW - In situ hybridisation
KW - N-Methyl-D-aspartate
UR - http://www.scopus.com/inward/record.url?scp=0031566003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031566003&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(97)00853-7
DO - 10.1016/S0304-3940(97)00853-7
M3 - Article
C2 - 9547169
AN - SCOPUS:0031566003
VL - 239
SP - 49
EP - 53
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -