MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts

Marieke L. Kuijjer, Heidi M. Namløs, Esther I. Hauben, Isidro MacHado, Stine H. Kresse, Massimo Serra, Antonio Llombart-Bosch, Pancras C W Hogendoorn, Leonardo A. Meza-Zepeda, Ola Myklebost, Anne Marie Cleton-Jansen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Conventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expression profiles of osteosarcoma cell lines and xenografts are still comparable to those of the primary tumor. Methods. We performed genome-wide mRNA expression profiling on osteosarcoma biopsies (n = 76), cell lines (n = 13), and xenografts (n = 18). Osteosarcoma can be subdivided into several histological subtypes, of which osteoblastic, chondroblastic, and fibroblastic osteosarcoma are the most frequent ones. Using nearest shrunken centroids classification, we generated an expression signature that can predict the histological subtype of osteosarcoma biopsies. Results: The expression signature, which consisted of 24 probes encoding for 22 genes, predicted the histological subtype of osteosarcoma biopsies with a misclassification error of 15%. Histological subtypes of the two osteosarcoma model systems, i.e. osteosarcoma cell lines and xenografts, were predicted with similar misclassification error rates (15% and 11%, respectively). Conclusions: Based on the preservation of mRNA expression profiles that are characteristic for the histological subtype we propose that these model systems are representative for the primary tumor from which they are derived.

Original languageEnglish
Article number66
JournalBMC Medical Genomics
Volume4
DOIs
Publication statusPublished - 2011

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Osteosarcoma
Heterografts
Cell Line
Neoplasms
Biopsy
Survival Rate
Messenger RNA
Transcriptome
Genome
Gene Expression
Bone and Bones

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Kuijjer, M. L., Namløs, H. M., Hauben, E. I., MacHado, I., Kresse, S. H., Serra, M., ... Cleton-Jansen, A. M. (2011). MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts. BMC Medical Genomics, 4, [66]. https://doi.org/10.1186/1755-8794-4-66

MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts. / Kuijjer, Marieke L.; Namløs, Heidi M.; Hauben, Esther I.; MacHado, Isidro; Kresse, Stine H.; Serra, Massimo; Llombart-Bosch, Antonio; Hogendoorn, Pancras C W; Meza-Zepeda, Leonardo A.; Myklebost, Ola; Cleton-Jansen, Anne Marie.

In: BMC Medical Genomics, Vol. 4, 66, 2011.

Research output: Contribution to journalArticle

Kuijjer, ML, Namløs, HM, Hauben, EI, MacHado, I, Kresse, SH, Serra, M, Llombart-Bosch, A, Hogendoorn, PCW, Meza-Zepeda, LA, Myklebost, O & Cleton-Jansen, AM 2011, 'MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts', BMC Medical Genomics, vol. 4, 66. https://doi.org/10.1186/1755-8794-4-66
Kuijjer, Marieke L. ; Namløs, Heidi M. ; Hauben, Esther I. ; MacHado, Isidro ; Kresse, Stine H. ; Serra, Massimo ; Llombart-Bosch, Antonio ; Hogendoorn, Pancras C W ; Meza-Zepeda, Leonardo A. ; Myklebost, Ola ; Cleton-Jansen, Anne Marie. / MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts. In: BMC Medical Genomics. 2011 ; Vol. 4.
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abstract = "Background: Conventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expression profiles of osteosarcoma cell lines and xenografts are still comparable to those of the primary tumor. Methods. We performed genome-wide mRNA expression profiling on osteosarcoma biopsies (n = 76), cell lines (n = 13), and xenografts (n = 18). Osteosarcoma can be subdivided into several histological subtypes, of which osteoblastic, chondroblastic, and fibroblastic osteosarcoma are the most frequent ones. Using nearest shrunken centroids classification, we generated an expression signature that can predict the histological subtype of osteosarcoma biopsies. Results: The expression signature, which consisted of 24 probes encoding for 22 genes, predicted the histological subtype of osteosarcoma biopsies with a misclassification error of 15{\%}. Histological subtypes of the two osteosarcoma model systems, i.e. osteosarcoma cell lines and xenografts, were predicted with similar misclassification error rates (15{\%} and 11{\%}, respectively). Conclusions: Based on the preservation of mRNA expression profiles that are characteristic for the histological subtype we propose that these model systems are representative for the primary tumor from which they are derived.",
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T1 - MRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts

AU - Kuijjer, Marieke L.

AU - Namløs, Heidi M.

AU - Hauben, Esther I.

AU - MacHado, Isidro

AU - Kresse, Stine H.

AU - Serra, Massimo

AU - Llombart-Bosch, Antonio

AU - Hogendoorn, Pancras C W

AU - Meza-Zepeda, Leonardo A.

AU - Myklebost, Ola

AU - Cleton-Jansen, Anne Marie

PY - 2011

Y1 - 2011

N2 - Background: Conventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expression profiles of osteosarcoma cell lines and xenografts are still comparable to those of the primary tumor. Methods. We performed genome-wide mRNA expression profiling on osteosarcoma biopsies (n = 76), cell lines (n = 13), and xenografts (n = 18). Osteosarcoma can be subdivided into several histological subtypes, of which osteoblastic, chondroblastic, and fibroblastic osteosarcoma are the most frequent ones. Using nearest shrunken centroids classification, we generated an expression signature that can predict the histological subtype of osteosarcoma biopsies. Results: The expression signature, which consisted of 24 probes encoding for 22 genes, predicted the histological subtype of osteosarcoma biopsies with a misclassification error of 15%. Histological subtypes of the two osteosarcoma model systems, i.e. osteosarcoma cell lines and xenografts, were predicted with similar misclassification error rates (15% and 11%, respectively). Conclusions: Based on the preservation of mRNA expression profiles that are characteristic for the histological subtype we propose that these model systems are representative for the primary tumor from which they are derived.

AB - Background: Conventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expression profiles of osteosarcoma cell lines and xenografts are still comparable to those of the primary tumor. Methods. We performed genome-wide mRNA expression profiling on osteosarcoma biopsies (n = 76), cell lines (n = 13), and xenografts (n = 18). Osteosarcoma can be subdivided into several histological subtypes, of which osteoblastic, chondroblastic, and fibroblastic osteosarcoma are the most frequent ones. Using nearest shrunken centroids classification, we generated an expression signature that can predict the histological subtype of osteosarcoma biopsies. Results: The expression signature, which consisted of 24 probes encoding for 22 genes, predicted the histological subtype of osteosarcoma biopsies with a misclassification error of 15%. Histological subtypes of the two osteosarcoma model systems, i.e. osteosarcoma cell lines and xenografts, were predicted with similar misclassification error rates (15% and 11%, respectively). Conclusions: Based on the preservation of mRNA expression profiles that are characteristic for the histological subtype we propose that these model systems are representative for the primary tumor from which they are derived.

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