Northern blots of total retinal RNA from a number of different vertebrate species were probed with a cDNA fragment corresponding to translated portions of bovine IRBP mRNA. A hybridizing band was detected in normal human retina (4·6 kb), Y-79 human retinoblastoma cells (4·4 kb), and in retinas of monkey (4·6 kb), guinea-pig (4·9 kb), mouse (6·1 kb), rat (two bands at 5·4 kb and 6·6 kb), rabbit (6·3 kb), cow (6·5 kb), and hamster (7·6 kb). Thus, the IRBP gene is expressed in the retinas of a wide variety of mammalian species. The mRNAs could be readily detected in about 20 μg of total RNA, suggesting that, in these species, IRBP message is relatively abundant. In contrast, only very weak hybridization was detected on northern blots of the three bird species examined (chicken, duck, quail). IRBP mRNA is thus relatively well expressed in mammals, but not in birds.
- interphotoreceptor retinoid-binding protein
ASJC Scopus subject areas
- Sensory Systems