mRNA for interphotoreceptor retinoid-binding protein (IRBP): Distribution and size diversity in vertebrate species

Lila N. Inouye, Adriana Albini, Gerald J. Chader, T. Michael Redmond, John M. Nickerson

Research output: Contribution to journalArticle

Abstract

Northern blots of total retinal RNA from a number of different vertebrate species were probed with a cDNA fragment corresponding to translated portions of bovine IRBP mRNA. A hybridizing band was detected in normal human retina (4·6 kb), Y-79 human retinoblastoma cells (4·4 kb), and in retinas of monkey (4·6 kb), guinea-pig (4·9 kb), mouse (6·1 kb), rat (two bands at 5·4 kb and 6·6 kb), rabbit (6·3 kb), cow (6·5 kb), and hamster (7·6 kb). Thus, the IRBP gene is expressed in the retinas of a wide variety of mammalian species. The mRNAs could be readily detected in about 20 μg of total RNA, suggesting that, in these species, IRBP message is relatively abundant. In contrast, only very weak hybridization was detected on northern blots of the three bird species examined (chicken, duck, quail). IRBP mRNA is thus relatively well expressed in mammals, but not in birds.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalExperimental Eye Research
Volume49
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Vertebrates
Retina
Messenger RNA
Northern Blotting
Birds
RNA
Quail
Ducks
Retinoblastoma
Cricetinae
Haplorhini
Mammals
Chickens
Guinea Pigs
Complementary DNA
Rabbits
interstitial retinol-binding protein
Genes

Keywords

  • interphotoreceptor retinoid-binding protein
  • mRNA
  • retina
  • vertebrate

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

mRNA for interphotoreceptor retinoid-binding protein (IRBP) : Distribution and size diversity in vertebrate species. / Inouye, Lila N.; Albini, Adriana; Chader, Gerald J.; Redmond, T. Michael; Nickerson, John M.

In: Experimental Eye Research, Vol. 49, No. 2, 1989, p. 171-180.

Research output: Contribution to journalArticle

Inouye, Lila N. ; Albini, Adriana ; Chader, Gerald J. ; Redmond, T. Michael ; Nickerson, John M. / mRNA for interphotoreceptor retinoid-binding protein (IRBP) : Distribution and size diversity in vertebrate species. In: Experimental Eye Research. 1989 ; Vol. 49, No. 2. pp. 171-180.
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AU - Nickerson, John M.

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