MS-analysis of SILAC-labeled MYC-driven B lymphoma cells overexpressing miR-17-19b

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Abstract

Micro RNAs (miRNAs) are small non-coding RNAs, which dampen gene expression by repressing translation and/or inducing degradation of target-mRNAs. Although the role of miR-17-19b (a truncated version of miR-17-92 cluster) is well documented in MYC-driven B cell lymphomagenesis, little is known about the function of the cluster in the maintenance of full-blown lymphomas. We employed SILAC-based quantitative proteomics to identify miR-17-19b targets upon a mild overexpression of the cluster in B cell lymphomas, established from λ-MYC transgenic mice. The proteomics data described in detail in this study, whose follow up analysis with MaxQuant algorithm is part of the recent publication (Mihailovich et al., 2015) [1], are deposited to the ProteomeXchange Consortium via the PRIDE partner repository, with the accession code PRIDE: PXD002810.

Original languageEnglish
Pages (from-to)349-353
Number of pages5
JournalData in Brief
Volume7
DOIs
Publication statusPublished - Jun 1 2016

Keywords

  • B cell lymphoma
  • Mass spectrometry
  • MiR-17-92
  • MiRNA targets
  • MYC
  • Quantitative proteomics
  • SILAC

ASJC Scopus subject areas

  • General
  • Education

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