Mst1 inhibits autophagy by promoting the interaction between beclin1 and Bcl-2

Yasuhiro Maejima, Shiori Kyoi, Peiyong Zhai, Tong Liu, Hong Li, Andreas Ivessa, Sebastiano Sciarretta, Dominic P. Del Re, Daniela K. Zablocki, Chiao Po Hsu, Dae Sik Lim, Mitsuaki Isobe, Junichi Sadoshima

Research output: Contribution to journalArticlepeer-review

Abstract

Here we show that Mst1, a proapoptotic kinase, impairs protein quality control mechanisms in the heart through inhibition of autophagy. Stress-induced activation of Mst1 in cardiomyocytes promoted accumulation of p62 and aggresome formation, accompanied by the disappearance of autophagosomes. Mst1 phosphorylated the Thr108 residue in the BH3 domain of Beclin1, which enhanced the interaction between Beclin1 and Bcl-2 and/or Bcl-xL, stabilized the Beclin1 homodimer, inhibited the phosphatidylinositide 3-kinase activity of the Atg14L-Beclin1-Vps34 complex and suppressed autophagy. Furthermore, Mst1-induced sequestration of Bcl-2 and Bcl-xL by Beclin1 allows Bax to become active, thereby stimulating apoptosis. Mst1 promoted cardiac dysfunction in mice subjected to myocardial infarction by inhibiting autophagy, associated with increased levels of Thr108-phosphorylated Beclin1. Moreover, dilated cardiomyopathy in humans was associated with increased levels of Thr108-phosphorylated Beclin1 and signs of autophagic suppression. These results suggest that Mst1 coordinately regulates autophagy and apoptosis by phosphorylating Beclin1 and consequently modulating a three-way interaction among Bcl-2 proteins, Beclin1 and Bax.

Original languageEnglish
Pages (from-to)1478-1488
Number of pages11
JournalNature Medicine
Volume19
Issue number11
DOIs
Publication statusPublished - Nov 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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