MTOR inhibitor rapamycin suppresses striatal post-ischemic LTP

Veronica Ghiglieri, Valentina Pendolino, Vincenza Bagetta, Carmelo Sgobio, Paolo Calabresi, Barbara Picconi

Research output: Contribution to journalArticle

Abstract

The two complexes of the mammalian target of rapamycin (mTOR), mTORC1 and mTORC2, have central functions in the integration of both extracellular and intracellular signals that are also critical players in the induction of post-ischemic long-term potentiation (i-LTP), a pathological form of plasticity inducible in striatal medium spiny neurons (MSNs) after a brief episode of in vitro ischemia. To evaluate the involvement of mTOR complexes during ischemia we analyzed the time course of i-LTP by intracellular recordings of MSNs from corticostriatal slices incubated with 1μM mTOR inhibitor rapamycin. Although rapamycin did not affect the amplitude and duration of ischemia-induced membrane depolarization it fully prevented i-LTP, leaving unaffected the capability to undergo activity-dependent LTP following high-frequency stimulation of corticostriatal fibers. The present results argue for a role of mTOR complex in i-LTP and suggest that rapamycin, by selectively blocking i-LTP, represents a promising therapeutic tool to limit cellular damage after ischemic brain insult.

Original languageEnglish
Pages (from-to)328-331
Number of pages4
JournalExperimental Neurology
Volume226
Issue number2
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Oxygen and glucose deprivation
  • Striatum
  • Stroke
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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