MUC5AC, cytokeratin 20 and HER2 expression and K-RAS mutations within mucinogenic growth in congenital pulmonary airway malformations

Giulio Rossi, Bernard Gasser, Giuliana Sartori, Mario Migaldi, Matteo Costantini, Maria Cecilia Mengoli, Silvia Piccioli, Alberto Cavazza, Francesco Rivasi

Research output: Contribution to journalArticle

Abstract

Aims: To analyse the expression of several mucins (MUC1, MUC2, MUC3, MUC5AC and MUC6), epidermal growth factor receptor (EGFR), v-erb-b2 erythroblastic leukaemia viral oncogene homologue 2 (HER2), thyroid transcription factor-1 (TTF-1), caudal type homeobox 2 (CDX2) and cytokeratin 20 (CK20), and the presence of mutations of EGFR, KRAS and HER2 in congenital pulmonary airway malformations (CPAM). Methods and results: Forty-one cases of CPAM and six pulmonary sequestrations were included. TTF-1 expression was observed in all cases but was not seen in mucinogenic growths in CPAM. CDX2 expression was completely negative. MUC1 expression was noted in 12 (29%) CPAM and in 33% sequestrations. MUC5AC was noted in only five cases (26%) by immunohistochemistry and was found in the mucinogenic proliferations of type 1 CPAM. No immunolabelling was noted for the other mucins. EGFR was expressed variably in almost all cases, while HER2 and CK20 was seen exclusively in the mucinogenic proliferations. All mucinous growths were characterized by KRAS mutations. No EGFR and HER2 gene alterations were identified. Conclusions: KRAS mutations and MUC5AC, CK20 and HER2 expression was seen in all mucinogenic proliferations, supporting the neoplastic nature of these cytologically bland growths. These findings emphasize the importance of complete surgical resection of such lesions.

Original languageEnglish
Pages (from-to)1133-1143
Number of pages11
JournalHistopathology
Volume60
Issue number7
DOIs
Publication statusPublished - Jun 2012

Keywords

  • Congenital airway malformation
  • EGFR
  • HER2
  • KRAS
  • Lung
  • Mucin

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

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