Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate

Claudia Devito; Jorma Hinkula; Rupert Kaul; Lucia Lopalco; Job J. Bwayo; Francis Plummer; Mario Clerici; Kristina Broliden

doi:10.1097/00002030-200009080-00006

Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate

Claudia Devito, Jorma Hinkula, Rupert Kaul, Lucia Lopalco, Job J. Bwayo, Francis Plummer, Mario Clerici, Kristina Broliden

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.

Original language	English
Pages (from-to)	1917-1920
Number of pages	4
Journal	AIDS (London, England)
Volume	14
Issue number	13
DOIs	https://doi.org/10.1097/00002030-200009080-00006
Publication status	Published - Sep 8 2000

Keywords

Antibodies
HEPS
HIV-1
IgA
Mucosa
Neutralization

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1097/00002030-200009080-00006

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Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate. / Devito, Claudia; Hinkula, Jorma; Kaul, Rupert; Lopalco, Lucia; Bwayo, Job J.; Plummer, Francis; Clerici, Mario; Broliden, Kristina.

In: AIDS (London, England), Vol. 14, No. 13, 08.09.2000, p. 1917-1920.

Research output: Contribution to journal › Article › peer-review

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title = "Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate",

abstract = "Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.",

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AU - Devito, Claudia

AU - Hinkula, Jorma

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AU - Lopalco, Lucia

AU - Bwayo, Job J.

AU - Plummer, Francis

AU - Clerici, Mario

AU - Broliden, Kristina

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N2 - Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.

AB - Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.

KW - Antibodies

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KW - Mucosa

KW - Neutralization

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Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate

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