Abstract
Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.
Original language | English |
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Pages (from-to) | 1917-1920 |
Number of pages | 4 |
Journal | AIDS (London, England) |
Volume | 14 |
Issue number | 13 |
DOIs | |
Publication status | Published - Sep 8 2000 |
Keywords
- Antibodies
- HEPS
- HIV-1
- IgA
- Mucosa
- Neutralization
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology