Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate

Claudia Devito, Jorma Hinkula, Rupert Kaul, Lucia Lopalco, Job J. Bwayo, Francis Plummer, Mario Clerici, Kristina Broliden

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals. Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG. Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples. Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)1917-1920
Number of pages4
JournalAIDS (London, England)
Volume14
Issue number13
DOIs
Publication statusPublished - Sep 8 2000

Keywords

  • Antibodies
  • HEPS
  • HIV-1
  • IgA
  • Mucosa
  • Neutralization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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