TY - JOUR
T1 - Mucosal immune environment in colonic carcinogenesis
T2 - CD80 expression is associated to oxidative DNA damage and TLR4-NFκB signalling
AU - Scarpa, Marco
AU - Cardin, Romilda
AU - Bortolami, Marina
AU - Kotsafti, Andromachi
AU - Scarpa, Maria Cristina
AU - Pozza, Anna
AU - Maran, Giorgia
AU - Picciocchi, Marika
AU - Ruffolo, Cesare
AU - D'Incà, Renata
AU - Sturniolo, Giacomo C.
AU - Castagliuolo, Ignazio
AU - Castoro, Carlo
AU - Angriman, Imerio
PY - 2013/1
Y1 - 2013/1
N2 - Background: CD80 has been thought to play an active role in immunosurveillance as it has been found to be up-regulated in ulcerative colitis (UC) patients with dysplasia. The aim of the present study was to analyse early events in UC-related and non-inflammatory carcinogenesis with reference to CD80 expression to clarify what stimuli are involved in its up-regulation in these patients. Patients and methods: Sixty-two patients affected with UC, UC with dysplasia, UC and cancer, colonic adenoma, or colonic cancer and 11 healthy subjects were enroled in our study. Tissue samples were taken from surgical specimens during colonic resection or during colonoscopy. Mucosal mRNA expression of Toll-like receptor-4 (TLR4) and nuclear factor-kappaB (NF-κB) was quantified with Real Time RT-PCR. TLR4, β-catenin and p53 expressions were analysed by immunohistochemistry. Mucosal levels of activated NF-κB were measured with immunometric assays while 8-Hydroxydeoxyguanosine (8-OHdG) levels were quantified by high-performance liquid chromatography with electrochemical detection (HPLC-ED). Non-parametric tests were used for statistical analysis. Results: 8-OHdG mucosal levels were higher in the patients with UC + dysplasia with respect to those in the patients with UC only (p = 0.03). CD80 mRNA mucosal levels were directly correlated with 8-OHdG mucosal levels (τ = 0.26, p = 0.04), TLR4 protein expression (τ = 0.45, p <0.01) and NF-κB mRNA expression and activity (τ = 0.24, p = 0.02; τ = 0.34, p = 0.02, respectively). CD80 protein expression, instead, was directly correlated with 8-OHdG mucosal levels (τ = 0.19, p = 0.05) and inversely correlated with TLR4 mRNA expression (τ = -0.25, p = 0.03). Conclusion: Oxidative DNA damage peaked in UC-related dysplasia and was found to be directly correlated to CD80 expression. The direct correlation between TLR4 protein expression and CD80 mRNA and the indirect correlation between CD80 protein and TLR4 mRNA expressions give substance to the hypothesis that they play a role in immunosurveillance. No significant correlations between CD80 expression and p53 and β-catenin accumulation during oncogenesis were, instead, observed.
AB - Background: CD80 has been thought to play an active role in immunosurveillance as it has been found to be up-regulated in ulcerative colitis (UC) patients with dysplasia. The aim of the present study was to analyse early events in UC-related and non-inflammatory carcinogenesis with reference to CD80 expression to clarify what stimuli are involved in its up-regulation in these patients. Patients and methods: Sixty-two patients affected with UC, UC with dysplasia, UC and cancer, colonic adenoma, or colonic cancer and 11 healthy subjects were enroled in our study. Tissue samples were taken from surgical specimens during colonic resection or during colonoscopy. Mucosal mRNA expression of Toll-like receptor-4 (TLR4) and nuclear factor-kappaB (NF-κB) was quantified with Real Time RT-PCR. TLR4, β-catenin and p53 expressions were analysed by immunohistochemistry. Mucosal levels of activated NF-κB were measured with immunometric assays while 8-Hydroxydeoxyguanosine (8-OHdG) levels were quantified by high-performance liquid chromatography with electrochemical detection (HPLC-ED). Non-parametric tests were used for statistical analysis. Results: 8-OHdG mucosal levels were higher in the patients with UC + dysplasia with respect to those in the patients with UC only (p = 0.03). CD80 mRNA mucosal levels were directly correlated with 8-OHdG mucosal levels (τ = 0.26, p = 0.04), TLR4 protein expression (τ = 0.45, p <0.01) and NF-κB mRNA expression and activity (τ = 0.24, p = 0.02; τ = 0.34, p = 0.02, respectively). CD80 protein expression, instead, was directly correlated with 8-OHdG mucosal levels (τ = 0.19, p = 0.05) and inversely correlated with TLR4 mRNA expression (τ = -0.25, p = 0.03). Conclusion: Oxidative DNA damage peaked in UC-related dysplasia and was found to be directly correlated to CD80 expression. The direct correlation between TLR4 protein expression and CD80 mRNA and the indirect correlation between CD80 protein and TLR4 mRNA expressions give substance to the hypothesis that they play a role in immunosurveillance. No significant correlations between CD80 expression and p53 and β-catenin accumulation during oncogenesis were, instead, observed.
KW - 8-OHdG
KW - CD80
KW - Colonic cancerogenesis
KW - Colorectal cancer
KW - Immunosurveillance
KW - NF-κB
KW - TLR4
KW - Ulcerative colitis
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U2 - 10.1016/j.ejca.2012.05.015
DO - 10.1016/j.ejca.2012.05.015
M3 - Article
C2 - 22704122
AN - SCOPUS:84871426580
VL - 49
SP - 254
EP - 263
JO - European Journal of Cancer
JF - European Journal of Cancer
SN - 0959-8049
IS - 1
ER -