MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line

Beate Rinner, Greta Gandolfi, Katharina Meditz, Marie-Therese Frisch, Karin Wagner, Alessia Ciarrocchi, Federica Torricelli, Raili Koivuniemi, Johanna Niklander, Bernadette Liegl-Atzwanger, Birgit Lohberger, Ellen Heitzer, Nassim Ghaffari-Tabrizi-Wizsy, Dagmar Zweytick, Iris Zalaudek

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few melanogenic cell lines retain their in vivo characteristics. In this work we present an intensively pigmented and well-characterized cell line derived from a highly aggressive NRAS mutated cutaneous melanoma, named MUG-Mel2. We present the clinical course, unique morphology, angiogenic properties, growth characteristics using in vivo experiments and 3D cell culture, and results of the exome gene sequencing of an intensively pigmented melanogenic cell line MUG-Mel2, derived from a cutaneous metastasis of an aggressive NRAS p. Q61R mutated melanoma. Amongst several genetic alterations, mutations in GRIN2A, CREBP, PIK3C2G, ATM, and ATR were present. These mutations, known to reinforce DNA repair problems in melanoma, might serve as potential treatment targets. The aggressive and fast growing behavior in animal models and the obtained phenotype in 3D culture reveal a perfect model for research in the field of NRAS mutated melanoma.

Original languageEnglish
Pages (from-to)2098
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - May 18 2017

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Melanoma
Cell Line
Mutation
Exome
Skin
DNA Repair
Animal Models
Cell Culture Techniques
Neoplasm Metastasis
Phenotype
Growth
Research
Genes
Neoplasms

Keywords

  • Journal Article

Cite this

MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line. / Rinner, Beate; Gandolfi, Greta; Meditz, Katharina; Frisch, Marie-Therese; Wagner, Karin; Ciarrocchi, Alessia; Torricelli, Federica; Koivuniemi, Raili; Niklander, Johanna; Liegl-Atzwanger, Bernadette; Lohberger, Birgit; Heitzer, Ellen; Ghaffari-Tabrizi-Wizsy, Nassim; Zweytick, Dagmar; Zalaudek, Iris.

In: Scientific Reports, Vol. 7, No. 1, 18.05.2017, p. 2098.

Research output: Contribution to journalArticle

Rinner, B, Gandolfi, G, Meditz, K, Frisch, M-T, Wagner, K, Ciarrocchi, A, Torricelli, F, Koivuniemi, R, Niklander, J, Liegl-Atzwanger, B, Lohberger, B, Heitzer, E, Ghaffari-Tabrizi-Wizsy, N, Zweytick, D & Zalaudek, I 2017, 'MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line', Scientific Reports, vol. 7, no. 1, pp. 2098. https://doi.org/10.1038/s41598-017-02197-y
Rinner, Beate ; Gandolfi, Greta ; Meditz, Katharina ; Frisch, Marie-Therese ; Wagner, Karin ; Ciarrocchi, Alessia ; Torricelli, Federica ; Koivuniemi, Raili ; Niklander, Johanna ; Liegl-Atzwanger, Bernadette ; Lohberger, Birgit ; Heitzer, Ellen ; Ghaffari-Tabrizi-Wizsy, Nassim ; Zweytick, Dagmar ; Zalaudek, Iris. / MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line. In: Scientific Reports. 2017 ; Vol. 7, No. 1. pp. 2098.
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AU - Wagner, Karin

AU - Ciarrocchi, Alessia

AU - Torricelli, Federica

AU - Koivuniemi, Raili

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AU - Liegl-Atzwanger, Bernadette

AU - Lohberger, Birgit

AU - Heitzer, Ellen

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AU - Zalaudek, Iris

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AB - NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few melanogenic cell lines retain their in vivo characteristics. In this work we present an intensively pigmented and well-characterized cell line derived from a highly aggressive NRAS mutated cutaneous melanoma, named MUG-Mel2. We present the clinical course, unique morphology, angiogenic properties, growth characteristics using in vivo experiments and 3D cell culture, and results of the exome gene sequencing of an intensively pigmented melanogenic cell line MUG-Mel2, derived from a cutaneous metastasis of an aggressive NRAS p. Q61R mutated melanoma. Amongst several genetic alterations, mutations in GRIN2A, CREBP, PIK3C2G, ATM, and ATR were present. These mutations, known to reinforce DNA repair problems in melanoma, might serve as potential treatment targets. The aggressive and fast growing behavior in animal models and the obtained phenotype in 3D culture reveal a perfect model for research in the field of NRAS mutated melanoma.

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