Multi-Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co-Cultured Human Mesenchymal Stem Cells

Sofia Amadori, Paola Torricelli, Silvia Panzavolta, Annapaola Parrilli, Milena Fini, Adriana Bigi

Research output: Contribution to journalArticle


A promising strategy for osteochondral interface regeneration consists in the development of hybrid scaffolds, composed of distinct but integrated layers able to mimic the different regions of cartilage and bone. We developed multi-layered scaffolds by assembling a gelatin layer with layers containing different amounts of gelatin and hydroxyapatite nanocrystals, and using a gelatin solution (as a glue) to stick layers together. The scaffolds exhibit a high, interconnected porosity and mechanical properties varying with composition along the thickness of the scaffolds up to values of compressive stress and modulus of about 1 and 14MPa, respectively. In vitro tests demonstrate that the different layers of the scaffolds promote chondrogenic and osteogenic differentiation of Human Mesenchimal Stem Cells (hMSC). Multi-layered, easily synthesized scaffolds made of gelatin and hydroxyapatite are able to promote chondrogenic and osteogenic differentiation of human mesenchymal stem cells.

Original languageEnglish
Pages (from-to)1535-1545
Number of pages11
JournalMacromolecular Bioscience
Issue number11
Publication statusPublished - Nov 1 2015



  • co-cultures
  • gelatin
  • hydroxyapatite
  • multi-layered scaffolds
  • osteochondral regeneration

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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