Abstract
A promising strategy for osteochondral interface regeneration consists in the development of hybrid scaffolds, composed of distinct but integrated layers able to mimic the different regions of cartilage and bone. We developed multi-layered scaffolds by assembling a gelatin layer with layers containing different amounts of gelatin and hydroxyapatite nanocrystals, and using a gelatin solution (as a glue) to stick layers together. The scaffolds exhibit a high, interconnected porosity and mechanical properties varying with composition along the thickness of the scaffolds up to values of compressive stress and modulus of about 1 and 14MPa, respectively. In vitro tests demonstrate that the different layers of the scaffolds promote chondrogenic and osteogenic differentiation of Human Mesenchimal Stem Cells (hMSC). Multi-layered, easily synthesized scaffolds made of gelatin and hydroxyapatite are able to promote chondrogenic and osteogenic differentiation of human mesenchymal stem cells.
Original language | English |
---|---|
Pages (from-to) | 1535-1545 |
Number of pages | 11 |
Journal | Macromolecular Bioscience |
Volume | 15 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 1 2015 |
Keywords
- co-cultures
- gelatin
- hydroxyapatite
- multi-layered scaffolds
- osteochondral regeneration
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry