Multi-sulfonated ligands on gold nanoparticles as virucidal antiviral for Dengue virus.

Antonella Zacheo, Jan Hodek, Dariusz Witt, Giuseppe Felice Mangiatordi, Quy K. Ong, Ozgun Kocabiyik, Nicoletta Depalo, Elisabetta Fanizza, Valentino Laquintana, Nunzio Denora, Danilo Migoni, Piotr Barski, Francesco Stellacci, Jan Weber, Silke Krol

Research output: Contribution to journalArticlepeer-review

Abstract

Dengue virus (DENV) causes 390 million infections per year. Infections can be asymptomatic or range from mild fever to severe haemorrhagic fever and shock syndrome. Currently, no effective antivirals or safe universal vaccine is available. In the present work we tested different gold nanoparticles (AuNP) coated with ligands ω-terminated with sugars bearing multiple sulfonate groups. We aimed to identify compounds with antiviral properties due to irreversible (virucidal) rather than reversible (virustatic) inhibition. The ligands varied in length, in number of sulfonated groups as well as their spatial orientation induced by the sugar head groups. We identified two candidates, a glucose- and a lactose-based ligand showing a low EC50 (effective concentration that inhibit 50% of the viral activity) for DENV-2 inhibition, moderate toxicity and a virucidal effect in hepatocytes with titre reduction of Median Tissue Culture Infectious Dose log10TCID50 2.5 and 3.1. Molecular docking simulations complemented the experimental findings suggesting a molecular rationale behind the binding between sulfonated head groups and DENV-2 envelope protein.
Original languageEnglish
Article number9052
JournalSci. Rep.
Volume10
Issue number1
DOIs
Publication statusPublished - Jun 3 2020

Fingerprint Dive into the research topics of 'Multi-sulfonated ligands on gold nanoparticles as virucidal antiviral for Dengue virus.'. Together they form a unique fingerprint.

Cite this